Study Biocompatibility and Osteogenic Differentiation Potential of Human Umbilical Cord Mesenchymal Stem Cells (hUCMSCs) with Gelatin Solvent

Abstract

The human umbilical cord is a source of numerous Mesenchymal Stem Cells (MSCs), making it as a potential source of allogeneic multipotent cell for bone tissue engineering. The aims of this study were to find: 1) Human Umbilical Cord Mesenchymal Stem Cells (hUCMSCs) phenotypic characterization, 2) The in-vitro osteogenic differentiation potential of hUCMSCs, 3) The cytotoxicity of gelatin solvent to hUCMSCs using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay. As a result, through characterization of hUCMSCs, the majority of target cells expressed specific MSCs markers, Cellular Differentiation (CD)73, smaller number of subpopulation expressed CD90 with only minimal subpopulation expressed CD105 and all negative MSCs markers. Osteoblastic differentiation was found in a significantly high number of cells when in vitro osteogenic differentiation of hUCMSCs with Alizarin Red staining was done. Biocompatibility analysis using the MTT assay showed that gelatin solvent and Alpha modification of minimum essential * Corresponding author.N. Hendrijantini et al. 421medium Eagle (α-MEM) was non-toxic for hUCMSCs in vitro. The study concluded that hUCMSCs isolated from human umbilical cord was capable of undergoing in vitro osteogenesis, indicating its potential as allogeneic stem cells for clinical application in bone tissue engineering.

Chondrogenic Differentiation Capacity of Human Umbilical Cord Mesenchymal Stem Cells with Platelet Rich Fibrin Scaffold in Cartilage Regeneration (In Vitro Study)

“…After the baby had delivered, 2 cm of umbilical cord was cut, washed in Phosphate Buffer Saline (PBS) three times to remove blood excess and blood clot, then soaked in Ringer Lactate solution containing 2.5 μg/mL gentamycin and 1000 U/ mL amphotericin B for 20 minutes. This technique was in accordance to Hendrijantini et al (2015) procurement technique.…”

Correlation between estrogen and alkaline phosphatase expression in osteoporotic rat model

“…Meanwhile, at the beginning of osteoblasts formation, ALP activity decrease, but during maturation process of osteoblasts, ALP activity will increase again. 21 Based on the results of Pearson correlation test, there was no significant correlation between estrogen and ALP in both of the normal group and the group of ovariectomized rats. This is due to estrogen levels in the systemic circulation affecting osteoblasts in the process of bone formation.…”

Virulence of periodontopathogens Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis: micro-computed tomography and inflammatory cytokine analysis

“…The method of IL1β expression painting was based on the UltraTek HRP Anti-Polyvalent (DAB) Staining System (Scytek, USA). The expressions of TNF-α and IL1β were observed under an Olympus CX-31 light microscope at 400x magnification [11,14].…”