Study efficacy of new model of derivative clonazepam on hypnotic, sedative, blood hematology and evaluation reproductive function in male mice

Abstract

Objectives This study includes the investigation of hypnotic and sedative effect of new generation agent synthesis from parent clonazepam and evaluates adverse effect on the reproductive system and an attempt to improve hypnotic effect and lower toxicity. Methods Sixteen male mice were randomly divided into four groups. The first day of treatment with single dose of 50 mg/kg intraperitoneal (IP) hypnotic effect then analgesic state was recorded in the 4th day of first week only after given 30 mg/kg orally, and then the experiment was persistent for 4 weeks to evaluate reproductive activity. Result The effect of diazepam on biochemical blood parameters showed that there is a slight increase in the WBC number of clonazepam group and 88 compound while there is no significant difference at the probability level 0.05 or 0.01, in 89 compound. RBC count in our result reveals a slight increase in 88 compound without significant difference than control. Our result shows significant increase in HB level in 88 compound to reach 13.18 ± 0.40 with no significant difference with control. PCV level reveals non-significant in all groups. Our results of the abnormal sperm morphology are shown after 4 weeks of the treatment. Conclusion clonazepam (T 1) and 88 compound (T 3) with high dose 50 mg/kg have good hypnotic action with complete muscle relaxant. In addition to all, agents have good analgesic effect but T 2 showed prominent result. Finally, clonazepam and its related agent 88, 89 compound reveal adverse effect on reproductive function but may be very slightly in 94 compound.