Ayurveda is one of the oldest systems of medicine that uses plants and their extracts for treatment and management of various diseased states. Nyctanthes arbour -tristis Linn. (Family Oleaceae), which has long been used traditionally for various medicinal purposes in the Indian sub continent. Each part of the plant has some important medicinal value and is thus commercially exploitable. It is now considered as a valuable source of several unique products for the medicines against various diseases and also for the development of some industrial products. The present study is to focus on the potential phyto-chemicals and pharmacological activity of plant N. arbor -tristis. In view of this, the study was designed to investigate for chemical constituents, anti-cancer and anti-inflammatory activities of solvent leaf extracts. Phytochemical screening revealed the presence of alkaloid, steroids etc. The extract exhibited significant anti-cancer and antiinflammatory properties and the observed biological activities in this study provide scientific validation of ethnomedicinal use of this plant.