Study of Cyclodextrin/Fluoroquinolone Inclusion Complexes by Capillary Electrophoresis

Abstract

Introduction:In the present work we evaluated the complexation role of cyclodextrins toward fl uoroquinolones in an attempt to assess their potential as new formulation additives for more effi cient fl uoroquinolone delivery and as selectors in capillary electrophoresis. Material and method: Guest-host interactions of two second generation quinolones, ciprofl oxacin and norfl oxacin with four cyclodextrins, beta-cyclodextrin (-CD), gamma-cyclodextrin (-CD) and two beta-cyclodextrin derivatives, 2-hydroxypropyl beta-cyclodextrin (HP--CD) and randomly methylated beta-cyclodextrin (RAMEB), were tested by capillary electrophoresis in borate running buffer. Experimental parameters like buffer concentration, pH, organic modifi er, voltage and cyclodextrin concentration have been varied for a better resolution. Results: In capillary zone electrophoresis ciprofl oxacin and norfl oxacin are migrating together, a difference in their migration times and thus separation occured by the addition of cyclodextrins. Conclusion: Our results suggest formation of inclusion complexes between fl uoroquinolones and cyclodextrins. Differences in their affi nity to host molecules resulted in separation of the two fl uoroquinolones.

Chiral selectors in CE: Recent development and applications (mid‐2014 to mid‐2016)

“…Moreover, when M-␤-CD was used as a CS, the complexation constants were higher for 10-hydroxywarfarin than for warfarin, and therefore, the former exerts stronger affinity to M-␤-CD and stronger stereoselective effect. RAMEB was used in CE as a CS for the enantioseparation of several analytes, such as omeprazole, amlodipine, and ofloxacin [51][52][53][54]. Briefly, Hancu et al [51] studied the chiral separation of omeprazole by using several neutral and negatively charged CDs.…”

“…RAMEB was also used for the chiral separation of sibutramine, and the method was validated based on linearity, precision, LOD, LOQ, and intra‐ and interday reproducibility . In a recent study , molecular modeling demonstrated that RAMEB interacts with ofloxacin enantiomers mainly by Van der Waals forces, and a small difference in the binding energies of the two enantiomers was observed. IR results indicated that a part of the oxazine ring is embedded in the CD cavity.…”