Study of direct electron transfer and enzyme activity of glucose oxidase on graphene surface

Electrochemical Biosensors Based on Nanostructured Carbon Black: A Review

“…However, DET is not easily achieved, because the enzyme active centre is protected by a glycoprotein shell, hindering the efficient electron tunneling [117]. In this sense, DET has been reported using nanostructured electrode architectures based on carbon nanomaterials, especially graphene derivatives [100,101,118] and MWCNTs [93,117]. DET between CB nanoparticles and hemoglobin (Hb) was diagnosed by the first time by Ma et al [84], which was an important advance in the use of CB nanoparticles for biosensing purposes.…”

Al-Based porous coordination polymer derived nanoporous carbon for immobilization of glucose oxidase and its application in glucose/O2 biofuel cell and biosensor

“…35 The effect of scan rate on the electrochemical performance of Naon/GOx/ PCP 1600 /GCE was shown in Fig. 5(C).…”

Immunosensing procedures for carcinoembryonic antigen using graphene and nanocomposites

“…Although frequent claims of DET of GOx on graphene surface have been made (Shan et al, 2009), the evidence is not at all persuasive or even unlikely if the experiment is conducted in the absence of oxygen, a natural mediator of GOx (Liang et al, 2015). In brief, DET between HRP and an electrode is difficult because the active sites of HRP are embedded inside a thick protein without the need for a mediator.…”

“…Direct electron transfer (DET) to HRP is well established in contrast to glucose oxidase (GOx) where DET is highly unlikely (Liang et al 2015). HRP is a very small enzyme (44 kDa) with the active heme group well exposed to the solution.…”