Study of electroporation effect on HT29 cell migration properties

Abstract

Pulse electric field exposures on human cancer cells have shown an alternative towards drug free wound treatments. The earlier investigations have shown interesting findings on usage of this electrical means generated via high voltage pulse generator system. Therefore, the aim of the study is to investigate the effect of pulse electric field on the migration properties of colon cell line HT29 towards the enhancement of wound healing process. The HT29 cells were treated with an electric field of 600V/cm for 500us after a wound model was created in vitro with the aid of a sterile needle. A time-lapse live imaging of the migration properties of the HT29 cell was carried out using integrated devices for live cell imaging that was equipped with CCD camera, temperature and CO 2 controllers and an inverted microscope. The result of cell migration properties showed that electroporation has an influence on migration speed of HT29 cell. The electroporated cells were found to close the wound completely in 105 minutes, at an average migration speed of 0.50 µm/min. Whereas the non-electroporated cells closed the wound in 240 minutes, at an average migration speed of 0.24 µm/min. Revealed by the live video microscopy, electroporation was also found to increase the proliferation rate of the HT29 cells. The cells in both treated and control collectively migrate in the direction of the wound closure. Thus, this can further be investigated for the use of pulse electric field in facilitating wound healing process.

Low amplitude pulse electric ϐield for elimination of unpleasant sensation associated with high amplitude electric ϐield for electrochemotherapy

“…Complete growth media used were RPMI1640 enriched with 10% fetal bovine serum (FBS) and 1% antibiotic (penicillin and streptomycin) which are all products of Gibco USA. The cells were cultured in a humidi ied incubator at 37• C, containing 5% CO 2 [32][33]. Cells were sub-cultured every 3 to 5 days whenever they reached 80% to 90% con luent [32].…”

“…The cells were cultured in a humidi ied incubator at 37• C, containing 5% CO 2 [32][33]. Cells were sub-cultured every 3 to 5 days whenever they reached 80% to 90% con luent [32].…”

Gerador de Marx Bipolar Baseado em Topologia Full-Bridge Aplicado à Eletropermeabilização Biológica

“…Aplicações práticas de eletropermeabilização biológica foram realizadas e publicadas em estudos nas últimas duas décadas. Elas foram utilizadas para analisar a migração celular em um processo de cura de um tecido intestinal [4]; na esterilização de amostras de suco de mirtilo [5]; no tratamento de câncer hepático e de pâncreas através da eletropermeabilização irreversível [6]; na desinfecção de água [7]; na inserção de moléculas de DNA em fetos de animais [8] e na destruição de parte de uma colônia do fungo patógeno Candida Albicans [9].…”

Evaluation of intracellular materials by biophotons released from cultured cells irradiated with atmospheric-pressure plasma

“…A USB photon counter (M014-01; Wavelength range: 230-700 nm; Dark count: average 50 CPS (Max: 100 CPS), OPTO-MECHATRONIX. Inc., Japan) and a stage-top culture system (Chamlide TC; Live Cell Instrument Co., Ltd., Korea) 21) for live-cell imaging was installed in a darkroom (B-S1-CN; SCIENTEX Inc., Japan). The experimental conditions are as follows.…”