Study of factors affecting the determination of total plasma 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate (SBD)–thiol derivatives by liquid chromatography

Rizzo, Vittoria; Montalbetti, Lorenza; Valli, M.; Bosoni, Tiziana; Scoglio, Enrico; Moratti, Remigio

doi:10.1016/s0378-4347(97)00547-1

Cited by 31 publications

(24 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A broad range of SBD-F concentration used for the derivatization can be found from the literature. Some were comparable to the one utilized in this study (Rizzo et al 1998;Frick et al 2003;Nolin et al 2007), while others were much higher. For example, 0.6% was used by Yoshida (Yoshida et al 2003), 0.3% used by Oe (Oe et al 1998) and 0.6 mg/ml by Dashalakis (Daskalakis et al 1996).…”

Section: ; Al-farawati and Van Den Berg 2001) For Quantitative Asupporting

confidence: 71%

“…The reagents themselves are not fluorescent. Therefore, the interferences from the excess reagent can be avoided (Imai et al 1994;Daskalakis et al 1996;Oe et al 1998;Rizzo et al 1998;Pfeiffer et al 1999;Uchiyama et al 2001;Abukhalaf et al 2002;Yoshida et al 2003).…”

Section: Discussionmentioning

confidence: 99%

“…Some works showed that TBP could decrease fluorescence signals (Rizzo et al 1998). Therefore, an extra extraction step using nhexane is usually required before derivatization (Kok et al 1997 …”

Section: Selection Of Reducing Reagents To Release Thiols On Thiopropmentioning

confidence: 99%

See 2 more Smart Citations

Reduced Organic Sulfur: Analyisis and Interaction with Mercury in the Aquatic Environment

Chen¹

View full text Add to dashboard Cite

Covalent affinity chromatography-based methods were attempted for analysis of ROS in environmental water samples. A method was developed for analysis of environmental thiols, by preconcentration using affinity covalent chromatographic column or solid phase extraction, followed by releasing of thiols from the thiopropyl sepharose gel using TCEP and analysis using HPLC-UV or HPLC-FL. Under the optimized conditions, the detection limits of the method using HPLC-FL detection were 0.45 and 0.36 nM for Cys and GSH, respectively. Our results suggest that covalent affinity methods are efficient for thiol enrichment and interference elimination, vii demonstrating their promising applications in developing a sensitive, reliable, and useful technique for thiol analysis in environmental water samples.The dissolution of mercury sulfide (HgS) in the presence of ROS and dissolved organic matter (DOM) was investigated, by quantifying the effects of ROS on HgS dissolution and determining the speciation of the mercury released from ROS-induced HgS dissolution. It was observed that the presence of small ROS (e.g., Cys and GSH) and large molecule DOM, in particular at high concentrations, could significantly enhance the dissolution of HgS. The dissolved Hg during HgS dissolution determined using the conventional 0.22 µm cutoff method could include colloidal Hg (e.g., HgS colloids) and truly dissolved Hg (e.g., Hg-ROS complexes). A centrifugal filtration method (with 3 kDa MWCO) was employed to characterize the speciation and reactivity of the Hg released during ROS-enhanced HgS dissolution. The presence of small ROS could produce a considerable fraction (about 40% of total mercury in the solution) of truly dissolved mercury (< 3 kDa), probably due to the formation of Hg-Cys or Hg-GSH complexes. The truly dissolved Hg formed during GSH-or Cys-enhanced HgS dissolution was directly reducible (100% for GSH and 40% for Cys) by stannous chloride, demonstrating its potential role in Hg transformation and bioaccumulation.

show abstract

Section: ; Al-farawati and Van Den Berg 2001) For Quantitative Asupporting

confidence: 71%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Reduced Organic Sulfur: Analyisis and Interaction with Mercury in the Aquatic Environment

Chen¹

View full text Add to dashboard Cite

show abstract

“…The SBD-F method has been used to determine low-molecular weight thiols, such as cysteine, homocysteine and glutathione, in plasma (Araki and Sako, 1987;Rizzo et al, 1998;Oe et al, 1998) and high-molecular weight metallothioneins (Miyairi et al, 1998).…”

Section: )mentioning

confidence: 99%

“…The modifications introduced to this procedure account mainly for the non-proteinic matrix analyzed in our study, as well as the smaller volumes used to economize the use of reagents. In addition, recent improvements reported in the literature (Rizzo et al, 1998;Oe et al, 1998;Miyairi et al, 1998) were taken into account as further explained below. This test applies to the determination of reduced and oxidized thiols in water samples by HPLC with fluorescence detection.…”

Section: Fluorimetric Assay Of Thiols With Sbd-fmentioning

confidence: 99%

Characterization and interactions of mercury, dissolved organic matter and organic sulfur in surface waters of the Florida Everglades

Sheils

Asunción²

View full text Add to dashboard Cite

Clinical relevance of plasma homocysteine levels in Taiwanese patients with coronary artery disease

Cheng

Lin

et al. 2008

BioFactors

View full text Add to dashboard Cite

Homocysteine (Hcy) has recently been considered as a risk factor for cardiovascular disease. Coronary artery disease (CAD) is the third most common cause of mortality in Taiwan. The objective of our present study is to delineate the relationship between the plasma Hcy level and CAD in Taiwanese using an improved enzymatic method. Blood samples were collected from 86 CAD patients, which include 38 patients with acute coronary syndrome (ACS) and 48 patients with stable CAD, and 89 controls. Plasma Hcy levels were measured by HPLC and enzymatic methods. Plasma Hcy levels were 8.27 +/- 1.74, 8.50 +/-1.88 and 11.76 +/- 4.58 muM in normal controls, patients with stable CAD and those with ACS, respectively. Plasma Hcy levels were elevated in the cohort of ACS patients, as compared with those of normal controls or patients with stable CAD. Within the CAD group, patients with the highest quartile Hcy level (10.18-23.73 muM) had a significantly higher odd ratio for ACS, as compared with those with the lowest quartile Hcy level after multivariate adjustment. Plasma Hcy concentration can be used as an independent risk factor of ACS, particularly for those with a history of CAD. Moreover, our improved enzymatic assay can be automated for large-scale screening of high-risk cohorts.

show abstract

Study of factors affecting the determination of total plasma 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate (SBD)–thiol derivatives by liquid chromatography

Cited by 31 publications

References 25 publications

Reduced Organic Sulfur: Analyisis and Interaction with Mercury in the Aquatic Environment

Reduced Organic Sulfur: Analyisis and Interaction with Mercury in the Aquatic Environment

Characterization and interactions of mercury, dissolved organic matter and organic sulfur in surface waters of the Florida Everglades

Clinical relevance of plasma homocysteine levels in Taiwanese patients with coronary artery disease

Contact Info

Product

Resources

About