Study of human prostate spheroids treated with zinc using X ray microfluorescence

Leitão, Roberta G.; Lopes, Ricardo Tadeu; Canellas, C.G.L.; Anjos, M.J.; Palumbo, Antônio; Nasciutti, Luiz Eurico; Santos, C. A. N.

doi:10.1109/memea.2014.6860133

Cited by 2 publications

(1 citation statement)

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“…Previously, it was shown that zinc biodistribution is not uniform, even in the same anatomical region of the prostate. Leitao and coworkers identified significant differences among transitional and peripheral zones 41 . However, to the best of our knowledge, the zinc-MT biodistribution in PCa is not known.…”

Section: Discussionmentioning

confidence: 99%

Prostate tumor attenuation in the nu/nu murine model due to anti-sarcosine antibodies in folate-targeted liposomes

Heger

Polanská

Rodrigo

et al. 2016

Sci Rep

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Herein, we describe the preparation of liposomes with folate-targeting properties for the encapsulation of anti-sarcosine antibodies (antisarAbs@LIP) and sarcosine (sar@LIP). The competitive inhibitory effects of exogenously added folic acid supported the role of folate targeting in liposome internalization. We examined the effects of repeated administration on mice PC-3 xenografts. Sar@LIP treatment significantly increased tumor volume and weight compared to controls treated with empty liposomes. Moreover, antisarAbs@LIP administration exhibited a mild antitumor effect. We also identified differences in gene expression patterns post-treatment. Furthermore, Sar@LIP treatment resulted in decreased amounts of tumor zinc ions and total metallothioneins. Examination of the spatial distribution across the tumor sections revealed a sarcosine-related decline of the MT1X isoform within the marginal regions but an elevation after antisarAbs@LIP administration. Our exploratory results demonstrate the importance of sarcosine as an oncometabolite in PCa. Moreover, we have shown that sarcosine can be a potential target for anticancer strategies in management of PCa.

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Section: Discussionmentioning

confidence: 99%