Study of Influential Factors on Oligosaccharide Formation by Fructosyltransferase Activity during Stachyose Hydrolysis by Pectinex Ultra SP-L

Montilla, Antonia; Olano, Agustı́n; Martínez‐Villaluenga, Cristina; Corzo, Nieves

doi:10.1021/jf202472p

Cited by 10 publications

(12 citation statements)

References 34 publications

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“…This effect was found by Das et al ., who reported that beyond a dose of 0.5% of β ‐galactosidase from B. circulans there was no further increase in GOS yield. The same effect was also observed by other authors for different enzymes and substrates . In our assays, since the yield differences between the reactions with 3 and 6 U mL −1 enzyme were negligible, the lower amount of enzyme was chosen to reduce the cost of operation.…”

Section: Resultssupporting

confidence: 80%

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Synthesis of prebiotic carbohydrates derived from cheese whey permeate by a combined process of isomerisation and transgalactosylation

et al. 2012

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Background Lactose from cheese whey permeate (WP) was efficiently isomerised to lactulose using egg shell, a food‐grade catalyst, and the subsequent transgalactosylation reaction of this mixture with β‐galactosidase from Bacillus circulans gave rise to a wide array of prebiotic carbohydrates derived from lactose and lactulose. Results Lactulose obtained by efficient isomerisation of WP (16.1% by weight with respect to the initial amount of lactose) showed great resistance to the hydrolytic action of β‐galactosidase from B. circulans, which preferentially hydrolysed lactose, acting as a galactosyl donor and acceptor. Lactulose had capacity as an acceptor, leading to the formation of lactulose‐derived oligosaccharides. The enzymatic synthesis was optimised by studying reaction conditions such as pH, temperature, time, enzyme concentration and carbohydrate concentration. The maximum formation of galactooligosaccharides with degrees of polymerisation from 2 to 4 was achieved after 5 h of reaction at pH 6.5 and 50 °C with 300 g kg−1 carbohydrates and 3 U mL−1 β‐galactosidase. Conclusion These findings indicate that the transgalactosylation of isomerised WP with β‐galactosidase from B. circulans could be a new and efficient method to obtain a mixture with 50% of potentially prebiotic carbohydrates composed of lactulose, and galactooligosaccharides derived from lactose and lactulose.

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Section: Resultssupporting

confidence: 80%

“…The same effect was also observed by other authors for different enzymes and substrates. 33,34 In our assays, since the yield differences between the reactions with 3 and 6 U mL −1 enzyme were negligible, the lower amount of enzyme was chosen to reduce the cost of operation. .…”

Section: Effects Of Enzyme and Substrate Concentrationsmentioning

confidence: 99%

Synthesis of prebiotic carbohydrates derived from cheese whey permeate by a combined process of isomerisation and transgalactosylation

et al. 2012

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“…Commercial enzyme preparations have been successfully used for the production of oligosaccharide mixtures to act as a prebiotic (Im et al 2016;Montilla et al 2011). Commercial cellulase preparation from Trichoderma longibrachiatum was used for large-scale production of chitooligosaccharides (Tegl et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Optimization of enzymatic hydrolysis of copra meal: compositions and properties of the hydrolysate

Thongsook

Chaijamrus

2018

J Food Sci Technol

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This study demonstrated that oligosaccharides from copra meal could be prepared by using a commercial enzyme preparation containing mannanase for use as a prebiotic. The conditions giving the highest hydrolysis rate of the copra meal by the enzyme were pH 4 and temperature of 40 °C with an enzyme to substrate ratio (E/S) of 1092.69 β-mannanase activity unit/g of the dried copra meal. Monosaccharide was the predominant product of the hydrolysis reaction followed by di- and tri-saccharide. Activated carbon treatment of the copra meal hydrolysate reduced the monosaccharide content resulting in 36.13% of monosaccharide, 54.26% of disaccharide and 9.61% of trisaccharide. All monosaccharides were eliminated by incubating the copra meal hydrolysate with for 48 h, which promoted the growth of, ,, ,, ,, , and while retarding the growth of the pathogenic bacteria, and.

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“…34 This different composition may be due to the fact that original α-galactosides could have been enzymatically treated with a fructosidase. Montilla et al (2011), 35 previously, found that this enzyme, under appropriate conditions, is able to completely eliminate the fructose from stachyose, forming manninotriose. The evolution in the content of di-, tri-and tetrasaccharides in the samples was highly dependent on the structure during the RSIE digestion treatment.…”

Section: Digestion Of Prebiotic Carbohydrates Using Rsiementioning

confidence: 97%

Evaluation of the impact of a rat small intestinal extract on the digestion of four different functional fibers

et al. 2020

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Study of Influential Factors on Oligosaccharide Formation by Fructosyltransferase Activity during Stachyose Hydrolysis by Pectinex Ultra SP-L

Cited by 10 publications

References 34 publications

Synthesis of prebiotic carbohydrates derived from cheese whey permeate by a combined process of isomerisation and transgalactosylation

Synthesis of prebiotic carbohydrates derived from cheese whey permeate by a combined process of isomerisation and transgalactosylation

Optimization of enzymatic hydrolysis of copra meal: compositions and properties of the hydrolysate

Evaluation of the impact of a rat small intestinal extract on the digestion of four different functional fibers

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