Study of interaction between Papaya ringspot virus HC-Pro and papaya (Carica papaya) proteins

Siriwan, Wanwisa; Takaya, Naoki; Roytrakul, Sittiruk; Chowpongpang, Srimek

doi:10.1007/s10327-014-0523-5

Cited by 1 publication

(2 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our findings confirm that networks of coat protein of potyvirus and host protein interaction are highly connected, with some proteins playing the role of hubs. However, interacted proteins results of this study did not directly connect with HC-Pro interacted protein that Siriwan et al (2014) reported. Bosque et al (2014) represented HC-Pro protein might interact with CP through CI, VPg and NIaPro or intermediary plant proteins.…”

Section: Discussioncontrasting

confidence: 85%

“…This approach can capture higher-order components of a larger complex, with the information being crucial to determine the function of a protein in plant cellular (Zhang et al 2010;Morris et al 2014). Previously, we analyzed the protein-protein interactions between 23 papaya (Carica papaya) proteins and PRSV HC-Pro protein using the AP-MS approach (Siriwan et al 2014). Recently, AP-MS has also been applied to explore the interaction of non-structure protein 1 of the dengue virus with the host cell proteins (Dechtawewat et al 2016).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Study of interaction between Papaya ringspot virus coat protein and infected Carica papaya proteins

Siriwan

Roytrakul

Chowpongpang

et al. 2021

Journal of Plant Interactions

Self Cite

View full text Add to dashboard Cite

Papaya ringspot virus (PRSV) causes the Papaya ringspot disease. Virus-host interactions appear to play a significant role in the replication, pathogenesis, and infection caused by the PRSV. PRSV coat protein is likely to be involved in the processes of RNA replication, aphid transmission, and cell-to-cell movement, which is closely related to the host cell proteins resulting in a PRSV infection. To identify the host proteins that interact with the CP in vitro, immune precipitation, insolution trypsin digestion, and LC-MS/MS were performed. Twenty-three identified proteins that interacted with the CP are involved in cellular metabolism, transcription, signal, translation, carbohydrate metabolism, protein metabolism, stress response, photosynthesis, nucleotide metabolism, respiration, and lipid metabolism processes. The search tool for interactions of chemicals (STITCH) results show that SWP (an RNA polymerase II transcription mediator) and MPPBETA (Mitochondrial processing peptidase) are involved in known plant defense mechanisms including transcription factors, cell division, hormones, stress, mitochondrial electron transferase, respiration, and proteasome. Therefore, analyzing virus-host protein interactions at a molecular level is important to build a better understanding of the virus replication mechanism and cellular responses mounted against viruses by the host defense system.

show abstract

Section: Discussioncontrasting

confidence: 85%