Study of interactions between carboxylated core shell magnetic nanoparticles and polymyxin B by capillary electrophoresis with inductively coupled plasma mass spectrometry

Abstract

In this work, interactions of carboxylated core shell magnetic nanoparticles with polymyxin B sulfate were studied by connecting capillary electrophoresis with inductively coupled plasma mass spectrometry. The interaction was probed by affinity mode of capillary electrophoresis with 25 mM phosphate buffer at physiological pH. 54 Fe, 56 Fe, 57 Fe, 34 S, and 12 C isotopes were used to monitor the migration of an electroosmotic flow marker and the interaction of the nanoparticles with polymyxin B. The analysis of interaction data showed two distinct interaction regions, one with low polymyxin B concentration, the second with high polymyxin B concentration. These regions differed in the strength of the interaction, 14.9 x 10 6 M-1 and 16.0 x 10 3 M-1 , and in the stoichiometry of 0.7 and 3.5, respectively. These differences can be explained by the decrease of electrostatic repulsion between nanoparticles caused by polymyxin B. This is also in agreement with the nanoparticles peak shapes: sharp for low polymyxin B concentrations and broad for high polymyxin B concentrations.

Electroosmotic flow modulation for improved electrokinetic preconcentration: Application to capillary electrophoresis of fluorescent magnetic nanoparticles

“…Their peak shapes and resolutions nevertheless are satisfactory for further characterization study. Compared to previously reported methods for CSMNPs detection [40,43], our CE-LEDIF method without LVSS allows an improvement of 3.5 times (compared to CE-MS) and 17 times (compared to CE-UV) respectively for the detection of the CSMNPs' magnetic cores. With the inclusion of the LVSS preconcentration step, a detection limit improvement of 340 was achieved compared to conventional CE-UV for this purpose.…”

“…Herein, we report for the first time a CE coupled with light-emitting-diode (LED) induced fluorescence detection approach (CE-LEDIF) for CSMNPs, offering significant improvement of detection sensitivity compared to the previously reported methods using UV or inductively coupled plasma mass spectrometry (ICP-MS) detection [40][41][42][43]. This was achieved with a novel way to perform on-line electrokinetic preconcentration of CSMNPs via modulation of the EO mobility (µEO) in fused silica capillaries without recourse to any coating nor viscous gel.…”

Salvage parenteral antibiotics for multidrug‐resistant (MDR) Gram‐negative bacteria; a fluorescamine‐based technique for ultrasensitive spectrofluorimetric measurement of Polymyxins; human plasma application

“…Considering spectroscopic methods, few ultraviolet (UV) spectrophotometric [ 8–12 ] and just two fluorimetric publications [ 13,14 ] , one for each antibiotic, have been reported previously. Furthermore, several chromatographic techniques such as high performance liquid chromatography (HPLC), [ 15–23 ] liquid chromatography (LC), [ 24–33 ] thin layer chromatography (TLC), [ 10,34 ] ultrahigh performance liquid chromatography (UPLC) [ 35–37 ] , and electrophoretic techniques [ 38–41 ] have been published. Conversely, microbiological assays [ 42–44 ] have been reported.…”

“…techniques such as high performance liquid chromatography (HPLC), [15][16][17][18][19][20][21][22][23] liquid chromatography (LC), [24][25][26][27][28][29][30][31][32][33] thin layer chromatography (TLC), [10,34] ultrahigh performance liquid chromatography (UPLC) [35][36][37] , and electrophoretic techniques [38][39][40][41] have been published. Conversely, microbiological assays [42][43][44] have been reported.…”

A CE-ICP-MS/MS method for the determination of superparamagnetic iron oxide nanoparticles under simulated physiological conditions

“…CE with a diode-array detector (DAD) was also utilized for monitoring interactions of Fe 3 O 4 NPs with bovine serum albumin [27]. In a recent report, CE-ICP-MS/MS was employed to study interactions between carboxylated core-shell magnetic nanoparticles and antibiotic (polymyxin B) in affinity capillary electrophoresis (ACE) mode, with the aim of developing a microdevice based on SPIONs to test bacterial resistance [28]. However, the experiments were conducted under one set of electrophoretic conditions only (no optimization trials reported) and were not directed towards quantitative analysis.…”