Study of Macrophages in BCG Granulomas in Different Compartments of the Mononuclear Phagocyte System

Архипов, С. А.; Shkurupy, V. A.; Соломатина, М. В.; Akhramenko, E. S.; Iljine, D. A.

doi:10.1007/s10517-013-1979-6

Cited by 5 publications

(8 citation statements)

References 13 publications

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“…We have previously shown that proportions ofILla-, TNF-a-, and GM-CSF-expressing macrophages from BCG granulomas, explanted in cultures one month after BCG vaccine infection, was more than lO-fold higher than the proportion of macrophages expressing the same cytokines and obtained from intact animals (11,16). The approximate two-fold decrease of these indicators was seen three months after infection.…”

Section: Resultsmentioning

confidence: 93%

“…The approximate two-fold decrease of these indicators was seen three months after infection. It was found that the proportions of BCG-infected mice splenic macrophages expressing IL-la, TNF-a and GM-CSF was several-fold higher than that of controls but significantly lower than in populations of macrophages from BCG granulomas (11,16). In this regard, it seemed interesting to elucidate the role of the investigated cytokines in antimycobacterial activity of macrophages of BCG granulomas.…”

Section: Resultsmentioning

confidence: 99%

“…Immunocytochemical studies of cultures of macrophages that have emigrated from granuloma and splenic macrophages were performed using an indirect immunocytochemical technique as previously described (11,16). The number of macrophages expressing IL-Ia, GM-CSF, and TNF-a was quantified using monoclonal antibodies (Becton Dickinson, USA): anti-mouse IL-Ia (Hamster Anti-Mouse, Isotype: Hamster IgGl,A), anti-mouse GM-CSF (Rat Anti-Mouse, Isotype: Rat IgG2a; clone MPI-22E9), anti-mouse TNF-a (Rat Anti-Mouse, Isotype: Rat IgGI; clone MP6-XT22).…”

Section: Methods Ofimmunocytochemical Analysis Ofmacrophages In Culturesmentioning

confidence: 99%

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In vitro Study of BCG Granuloma Macrophage Morphofunctional Status

et al. 2014

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We studied the morphofunctional and cytophysiological status of macrophages emigrating from BCG granulomas forming in spleen and free splenic macrophages that are not associated with granulomas. The experimental BCG granulomatosis was induced by intravenous injection of male BALB/c mice with BCG vaccine mycobacteria. The number of granulomas in spleen, their diameter, the proportion of granuloma macrophages with mycobacteria, the number of mycobacteria in granuloma macrophages, the proportion of live bacteria in granuloma macrophages and the number ofgranulomas macrophages capable of expressing IL-la, TNF-a, GM-CSF were evaluated. BCG granulomas were explanted in cultures in vitro. Fractions, containing free splenic macrophages from BCG-infected animals, were explanted in separate cultures in vitro. The phagocytic activity of macrophages that migrated from BCG granulomas explanted in cultures one month after mycobacterial infection of mice, was much higher than those of splenic macrophages of intact mice. The phagocytic activity of free macrophages and macrophages from granulomas decreased with time after infection. By contrast, the antimycobacterial activity offree splenic macrophages and macrophages from BCG granulomas increased with time after infection. The correlational analysis showed that there are different correlational relationships between the number of granuloma macrophages expressing IL-la, TNF-a, GM-CSF and phagocytic activity ofmacrophages from BCG granulomas. The results ofthe study are important for understanding the molecular and ceUularmechanisms of development ofchronic granulomatous inflammation induced by mycobacterial infection.

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Section: Resultsmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Methods Ofimmunocytochemical Analysis Ofmacrophages In Culturesmentioning

confidence: 99%

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In vitro Study of BCG Granuloma Macrophage Morphofunctional Status

et al. 2014

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“…Furthermore, carbon from various sugars and fatty acids are extracted by Mtb in host cells via its major enzymes, such as the polyphosphate glucokinase, isocitrate lyases (ICL1 and ICL2), and the phosphoenolpyruvate carboxykinase ( 37 – 39 ). Mtb favors the differentiation of macrophages toward M2 subtype ( 40 ). By contrast, it impairs the formation of M1 macrophages.…”

Section: Evasion Strategies Adopted By Mtb To Counmentioning

confidence: 99%

Reinforcing the Functionality of Mononuclear Phagocyte System to Control Tuberculosis

et al. 2018

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The mononuclear phagocyte system (MPS) constitutes dendritic cells, monocytes, and macrophages. This system contributes to various functions that are essential for maintaining homeostasis, activation of innate immunity, and bridging it with the adaptive immunity. Consequently, MPS is highly important in bolstering immunity against the pathogens. However, MPS is the frontline cells in destroying Mycobacterium tuberculosis (Mtb), yet the bacterium prefers to reside in the hostile environment of macrophages. Therefore, it may be very interesting to study the struggle between Mtb and MPS to understand the outcome of the disease. In an event when MPS predominates Mtb, the host remains protected. By contrast, the situation becomes devastating when the pathogen tames and tunes the host MPS, which ultimately culminates into tuberculosis (TB). Hence, it becomes extremely crucial to reinvigorate MPS functionality to overwhelm Mtb and eliminate it. In this article, we discuss the strategies to bolster the function of MPS by exploiting the molecules associated with the innate immunity and highlight the mechanisms involved to overcome the Mtb-induced suppression of host immunity. In future, such approaches may provide an insight to develop immunotherapeutics to treat TB.

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“…The expressions of different cytokine genes were examined for studying the macrophage polarization (Martinez and Gordon, 2014;Donates, 2014). There were some published articles about different aspects of BCG vaccine like its effect on classical macrophage polarization (Iqbal and Hussain, 2014;Oliveira, 2013;Lardone et al, 2013;Arkhipov et al, 2013;Nadeev et al, 2008;Potapova and Shkurupiy, 2008) and the effect of hydatid cyst fluid on alternatively activation of macrophages (Amri and Touil-Boukoffa, 2013). The purpose of this study was to investigate the gene expression system of TNFα and IFNγ for the group which was exposed to BCG particles and also, studying the expression of IL4 and TGFβ genes in macrophages which were exposed to fertile hydatid cyst fluid.…”

Section: Introductionmentioning

confidence: 99%

Assessing the Impact of BCG Vaccine and Fertile Hydatid Cyst Fluid in Classical and Alternative Macrophage Polarization

Jalili¹,

Moslemi²,

Izadi³

et al. 2015

Research J. of Microbiology

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Classical macrophages (M1) and alternative macrophages (M2) are responsible for various functions in order to maintain homeostasis. The BCG vaccine and hydatid cyst fluid can be examples of stimulants which can cause M1 and M2 macrophage polarization. Evaluating the expression of markers such as IFNγ and TNFα for M1 phenotype and TGFβ and IL4 for M2 phenotype is one of the confirmatory ways of polarization. The purpose of this study is to verify the polarization of macrophages which was induced by BCG vaccine and hydatid cyst fluid by studying the gene expression of aforementioned markers. Mononuclear normal human peripheral blood cells were separated by using ficoll 1077 and gradient concentration. Monocyte populations were separated by adhesion technique from cultured cells in complete tissue culture medium. Monocyte derived macrophages were treated by BCG intravesical vaccine and fertile hydatid cyst fluid for 8 h. The RT-PCR was performed to confirm the polarization. The expression of amplified genes were compared with control groups. Amplification of TNFα and IFNγ genes in M1 and TGFβ and IL4 in M2 group confirmed the polarization procedure. Also, lack of PCR product in negative control samples indicates either the absence or a very low expression of this gene in control group. The ability of these stimulants in macrophage polarization can be used in experimental studies. It is also possible to take advantage of this model to achieve useful goals in cell therapy and develop more efficient therapeutic methods in inflammatory diseases and cancer immunotherapy.

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Study of Macrophages in BCG Granulomas in Different Compartments of the Mononuclear Phagocyte System

Cited by 5 publications

References 13 publications

In vitro Study of BCG Granuloma Macrophage Morphofunctional Status

In vitro Study of BCG Granuloma Macrophage Morphofunctional Status

Reinforcing the Functionality of Mononuclear Phagocyte System to Control Tuberculosis

Assessing the Impact of BCG Vaccine and Fertile Hydatid Cyst Fluid in Classical and Alternative Macrophage Polarization

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