Study of Multidrug Resistance Pattern among Escherichia coli isolated from patients with Urinary tract infection

Abstract

Objective: Urinary tract infections (UTIs) are some of the most common bacterial infections encountered in community and cause of significant morbidity and high medical cost. Escherichia coli is the most common pathogen responsible for the majority of UTI infections. Antimicrobial drugs have been routinely prescribed for the empirical treatment of UTIs which has led to a dramatic increase in antibiotic resistance pattern of E. coli. The aim of the present study was to analyze the multidrug resistance (MDR) patterns of E. coli isolated from UTI patients.Methods: A total of 80 urine samples collected from the patients suspected of having UTI attending Maharishi Markandeshwar Institute of Medical Sciences and Research, Mullana, Ambala, were cultured using standard microbiological techniques. Antibiotic susceptibility testing of E. coli was done using minimum inhibitory concentration (MIC). MIC of tetracycline, doxycycline, azithromycin, erythromycin, ciprofloxacin, levofloxacin, ampicillin, amoxicillin, and amikacin was done by agar dilution method. Results:Of the total 46 isolates contributing 33 females and 13 males were confirmed as E. coli. About 51.34% of the female patients belonged to the age group 21-40 years and 53.84% of the male population belonged to 41-80 years were found to be more susceptible to UTI infection. All isolates confirmed as E. coli were found to be MDR. 80% of the isolates exhibited MICs higher than 1000 mg/L against β-lactams. 20% of the E. coli isolates exhibited MICs higher than 1000 mg/L against ciprofloxacin, amikacin, and erythromycin. 23% and 95% of E. coli isolates exhibited MICs <128 mg/L against doxycycline and levofloxacin, respectively. Conclusion:The present study revealed the decreased susceptibility of the E. coli to all drugs. E. coli resistance profile to β-lactams, quinolones, macrolides, tetracyclines, and aminoglycosides were also found to be quite high in this study emphasizing the need to educate public about appropriate use of antibiotics.

The Prevalence Of Uropathogenic Escherichia Coli Strains Among Outpatients With Urinary Tract Infection In Zakho City, Iraq

“…[20] While other studies reported different results such as: in India they reported the highest rate(51.15%) of E. coli infection among females aged 21-40 years, while it was 53.84% among males aged 40 to 80 years. [16] Over all ages, the risk of UTI infection in females was higher than that of males. [42] This could be attributed to a variety of factors, such as; the anatomical structure of the urinary tract, sexual intercourse, personal hygiene and number of pregnancies.…”

Antibiotic Resistance Pattern of Uropathogens among Non Pregnant Women: A Hospital based Cross Sectional Study from Odisha

“…Bacterial strains in spreaded plated were allowed to grow for 18-24 hours at 37°C, aerobically. 24 The zones of inhibition around each disc were measured and compared with the zone diameter as per the standards of the clinical laboratory standards institute (CLSI) guidelines. 25 Escherichia coli 10974 was obtained from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ), Germany.…”