The defected acoustic band gap materials are promising a new generation of sensing technology based on layered cavities. We introduced a novel 1D defected phononic crystal (1D-DPC) as a high-sensitive gas sensor based on the Fano resonance transmitted window. Our designed (Lead–Epoxy) 1D-DPC multilayer has filled with a defect layer with different gases at different temperatures. In this study, Fano resonance—based acoustic band gap engineering has used to detect several gases such as O2, CO2, NH3, and CH4. For the first time, Fano resonance peaks appeared in the proposed gas sensor structures which attributed to high sensitivity, Q-factor, and figure-of-merit values for all gases. Also, the relation between the Fano resonance frequency and acoustic properties of gases at different temperatures has been studied in detail. The effect of the damping rate on the sensitivity of the gas sensor shows a linear behavior for CO2, O2, and NH3. Further, we introduced the effect of temperature on the damping rate of the incident waves inside the 1D-DPC gas sensor. The highest sensitivity and figure of merit were obtained for O2 of 292 MHz/(kg/m3) and 647 m3/Kg, respectively. While the highest figure-of-merit value of 60 °C−1 at 30 °C was attributed to O2. The transfer matrix method is used for calculating the transmission coefficient of the incident acoustic wave. We believe that the proposed sensor can be experimentally implemented.