Study of physical processes occurring in serum-containing and polymer-based serum-free cryoprotective media
O. V. Pakhomov,
T. M. Gurina,
A. L. Polyakova
et al.
Abstract:To investigate the mechanisms of cryoprotection with compositions that were supplemented with polymers (dextran [Dex], hydroxyethyl starch [HES], polyethylene glycol [PEG]) or fetal bovine serum [FBS]). Methods. Differential scanning calorimetry (DSC) and thermomechanical analysis (TMA) of cryoprotective media containing serum, as well as media that do not contain serum or its components but include polymers. Results. The combinations of dimethyl sulfoxide with FBS or polymers in cryoprotective media were able… Show more
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