Study of Polymerized Hemoglobin in Experiment

Abstract

Lyophilized polymerized hemoglobin has no group specificity and does not damage the kidney after 2 days of storage; its T/2 is 14-16 hours. P50 of solution prepared on the hemoglobin basis is 24-28 torr at 37 degrees C and pCO2, 40 torr in 0.05 M Tris-buffer, pH 7.4. As was shown in models of hemorrhagic shock in dogs, the hemodynamic properties of the solutions is such that it is capable of increasing the cardiac output and maintains the arterial pressure at the level close to initial one. Analysis of the oxygen expenditure suggests the expediency of using the solution in doses of 1 gr/kg of body weight. The increase in oxygen capacity of the solutions administered in the said dose is the result of increase in the hemoglobin concentration in blood plasma. Contribution of the solution to blood oxygen capacity is 12-13%. The solution of lyophilized modified hemoglobin can be used for correction of both hemodynamic disorders and decreased oxygen delivery to tissues in hemorrhagic shock.