Study of Polyphenol Content and Antioxidant Capacity of Myrianthus Arboreus (Cecropiaceae) Root Bark Extracts

Kasangana, Pierre Betu; Haddad, Pierre S.; Stevanović, Tatjana

doi:10.3390/antiox4020410

Cited by 88 publications

(65 citation statements)

References 39 publications

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“…It is generally proven that the antioxidant activity of plants depends on the amounts of biologically active compounds (flavonoids, phenolics, phenylpropanoids, etc.). Generally, the higher the TPC and TFC, the stronger antioxidant and antifungal activities reported [16,18,26]. In the current study, the various solvent extracts of HFF were taken for analysis, where AE showed the higher antioxidant activity in DPPH radical-scavenging assay, and EAE exhibited the stronger antioxidant activities in ABTS radical-scavenging and the FRAP assay.…”

Section: In Vitro Antioxidant Activities Of Hff Extractsmentioning

confidence: 86%

Antioxidant, Antifungal Activities of Ethnobotanical Ficus hirta Vahl. and Analysis of Main Constituents by HPLC-MS

et al. 2020

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The medicinal and edible plant, Ficus hirta Vahl. (also called hairy fig), is used for the treatment of constipation, inflammation, postpartum hypogalactia, tumors, and cancer. There is an urgent need for scientific evaluation to verify the pharmacological properties of F. hirta. Therefore, in vitro assays evaluated the antioxidant and antifungal activities of various solvent extracts of hairy fig fruits (HFF). HFF extracts had abundant antioxidant components for a significant amount of total phenolic (TPC) and flavonoid contents (TFC) (TPC from 17.75 ± 0.52 to 85.25 ± 1.72 mg gallic acid/g dw and TFC from 15.80 ± 0.59 to 144.22 ± 8.46 mg rutin/g dw, respectively). The ethyl acetate extract (EAE) and acetone extract (AE) of HFF demonstrated potent antioxidant activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH) (IC50 values of 2.52 and 2.02 mg/mL, respectively) and ABTS radicals (IC50 values of 3.06 and 9.26 mg/mL, respectively). Moreover, the AE with a high TFC showed a prominent in vitro and in vivo antifungal activity against Penicillium italicum, causing citrus blue mold. Eighteen metabolites were identified or putatively identified from six HFF extracts. Current findings indicated that HFF extracts had significant antioxidant and antifungal activities and could potentially be used as an alternative agent for the preservation of agricultural products.

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Section: In Vitro Antioxidant Activities Of Hff Extractsmentioning

confidence: 86%

Antioxidant, Antifungal Activities of Ethnobotanical Ficus hirta Vahl. and Analysis of Main Constituents by HPLC-MS

et al. 2020

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“…Natural antioxidants have been studied extensively since they can protect cells from such oxidative damage and are assumed to be less toxic than synthetic antioxidants, such as butylated hydroxyanisole (BHA) and butylated hydroxyltoluene (BHT). However, they have been reported to cause liver damage and cancer 8 . In the present study, three medicinal plants viz.…”

Section: Introductionmentioning

confidence: 99%

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2018

IJPSR

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Plants have been used in traditional medicine for several thousand years since which may have been described to have curative value. Terminalia chebula (TC), Terminalia arjuna (TA) and Holarrhena antidysenterica (HA) are very important medicinal plants in the traditional Indian systems of Ayurveda and Siddha. In this work, fruit of Terminalia chebula, bark of Terminalia arjuna and Holarrhena antidysenterica were selected for experiments. 80% methanolic crude extracts of these plants were fractionated by diethyl ether and ethyl acetate. Diethyl ether fraction of 80% methanolic extract (DM) and ethyl acetate fraction of 80% methanolic extract (EM) of all the three plants were compared for their phytochemical, antioxidant and cytotoxicity to find out their therapeutic potential. The phenolic content was determined by Folin-Ciocalteu method and was found to be higher in Terminalia chebula extracts (>36 mg GAE/g)). The antioxidant activity was assayed through some in-vitro methods such as DPPH radical scavenging assay, phosphomolybdenum method and reducing power assay. Antioxidant activity was also found to be highest in Terminalia chebula extracts. Cytotoxicity of extracts was determined by the MTT assay using HeLa cell lines which showed that the increase in concentration of the extract increases the cell death. All the extracts showed moderate cytotoxicity with IC 50 values ranging from 147.91 to 1701 µg/ml. These findings showed that extracts from Terminalia chebula is a potential source of natural antioxidants and could be good alternatives to synthetic antioxidants in pharmaceutical industries. QUICK RESPONSE CODE

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“…[51,52] 100 lM of DPPH in ethanol was prepared and mixed with GA, ECG, and EGCG such that the final concentration of the polyphenol is 100 lM with ascorbic acid as the control. The reaction mixture was then incubated in the dark for 30 min at room temperature.…”

Section: Antioxidant Activity By Dpph (22-diphenyl-1-picrylhydrazymentioning

confidence: 99%

β‐cyclodextrin encapsulated polyphenols as effective antioxidants

et al. 2017

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Formation of dityrosine (DT) cross-linkages in proteins is one of the most widely used markers of oxidative stress. Ribonuclease A (RNase A) has 6 Tyr residues and shows a characteristic DT fluorescence peak upon oxidation in addition to major changes in its secondary structure. DT formation can be prevented by using polyphenols (GA, ECG, and EGCG) which are known to have strong antioxidant activity. However, it has been observed that ECG and EGCG initiate protein oligomerization due to protein-polyphenol cross-linkages. To prevent the formation of such crosslinkages we have used b-cyclodextrin (b-CD) to encapsulate the polyphenols and studied its antioxidant properties along with that of free polyphenols. The polyphenol/b-cyclodextrin (b-CD) inclusion complexes not only prevent DT formation but also reduce protein oligomerization. This may be attributed to the fact that the quinone forming rings of ECG and EGCG become encapsulated in the cavity of b-CD and are no longer available for protein cross-linking.b-cyclodextrin encapsulation, dityrosine, oxidative stress, polyphenols, protein oligomerization | I N TR ODU C TI ONBiological aging in mammalian cells is a direct indication of oxidative stress in proteins. [1] These oxidatively modified forms of proteins are generated due to the presence of reactive oxygen species (ROS) such as hydroxyl and superoxide free radicals. [2,3] Persulfates, a key ingredient in flour, are a source of sulfate (SO 2 4 ) free radicals under neutral pH. [4,5] Due to the higher oxidizing power of the sulfate radical (E 0 5 12.60 V) and longer half-life period over the OH radical, it has drawn attention in recent times as a powerful oxidizing agent. [6][7][8] Many chemical and cosmetic industries require persulfates as their key components [9] and workers often suffer from skin and respiratory diseases due to hypersensitive reactions. [10] Generally, the lower thermal energy at room temperature (25-508C) is not sufficient enough to activate persulfate, but generation of SO 2 4 radical is facilitated in presence of metal cations. Since proteins tend to lose their structure and function at high temperatures, [11] we have carried out the oxidation of ribonuclease A (RNase A) in presence of persulfate using cobalt (II) ion at 378C. [12] Cobalt is an integral component of vitamin B12 and thus physiologically found in most tissues mainly in liver and kidney. [13,14] Gill et al., reported the formation of oligomeric species (dimer, trimer, tetramer) when RNase A was exposed to KHSO 5 in presence of metal ions such as Ni 21 and Cu 21 and the high molecular weight species were identified using SDS-PAGE. [12] An important biomarker of oxidative stress is the dityrosine moiety generated due to the covalent linkage of two Tyr residues. [15] Dityrosine bond formation is an indication of aging, and disease related problems since it generally occurs during amyloid fibril formation, [16] Parkinson disease, [17] cerebrospinal fluid damage, [18] and also in the cataract of the eye lens. [19,20] D...

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Study of Polyphenol Content and Antioxidant Capacity of Myrianthus Arboreus (Cecropiaceae) Root Bark Extracts

Cited by 88 publications

References 39 publications

Antioxidant, Antifungal Activities of Ethnobotanical Ficus hirta Vahl. and Analysis of Main Constituents by HPLC-MS

Antioxidant, Antifungal Activities of Ethnobotanical Ficus hirta Vahl. and Analysis of Main Constituents by HPLC-MS

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β‐cyclodextrin encapsulated polyphenols as effective antioxidants

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