Study of renal function matching between banna minipig inbred line and human

Sun, Xiaorong; Zhang, L.; Cheng, Jun; Li, S.; Wang, Q.; Wei, Qing; Zeng, Yun‐Yun; Zeng, Renpan; Li, Y.

doi:10.1016/j.transproceed.2004.07.060

Cited by 7 publications

(4 citation statements)

References 9 publications

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“…The BMI was developed through more than 20 generations with high inbreeding coefficients [4–6]. BMI is considered as an ideal source for pig to human xenotransplantation to solve the serious shortage of donor organs [7–10]. …”

Section: Introductionmentioning

confidence: 99%

Highly Efficient Generation of GGTA1 Biallelic Knockout Inbred Mini-Pigs with TALENs

et al. 2013

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Inbred mini-pigs are ideal organ donors for future human xenotransplantations because of their clear genetic background, high homozygosity, and high inbreeding endurance. In this study, we chose fibroblast cells from a highly inbred pig line called Banna mini-pig inbred line (BMI) as donor nuclei for nuclear transfer, combining with transcription activator-like effector nucleases (TALENs) and successfully generated α-1,3-galactosyltransferase (GGTA1) gene biallelic knockout (KO) pigs. To validate the efficiency of TALEN vectors, in vitro-transcribed TALEN mRNAs were microinjected into one-cell stage parthenogenetically activated porcine embryos. The efficiency of indel mutations at the GGTA1-targeting loci was as high as 73.1% (19/26) among the parthenogenetic blastocysts. TALENs were co-transfected into porcine fetal fibroblasts of BMI with a plasmid containing neomycin gene. The targeting efficiency reached 89.5% (187/209) among the survived cell clones after a 10 d selection. More remarkably 27.8% (58/209) of colonies were biallelic KO. Five fibroblast cell lines with biallelic KO were chosen as nuclear donors for somatic cell nuclear transfer (SCNT). Three miniature piglets with biallelic mutations of the GGTA1 gene were achieved. Gal epitopes on the surface of cells from all the three biallelic KO piglets were completely absent. The fibroblasts from the GGTA1 null piglets were more resistant to lysis by pooled complement-preserved normal human serum than those from wild-type pigs. These results indicate that a combination of TALENs technology with SCNT can generate biallelic KO pigs directly with high efficiency. The GGTA1 null piglets with inbred features created in this study can provide a new organ source for xenotransplantation research.

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Section: Introductionmentioning

confidence: 99%

Highly Efficient Generation of GGTA1 Biallelic Knockout Inbred Mini-Pigs with TALENs

et al. 2013

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“…Currently, the most widely used mini-swine breeds in U.S. research laboratories and contract research organizations include the Hanford™ (Ho et al 2010), Sinclair™ (Misfeldt and Grimm 1994), Yucatan™ (Panepinto and Phillips 1986), and Göttingen (Simianer and Kohn 2010). Other mini-swine breeds such as Tibetan (Fan et al 2003), Banna (Sun et al 2004), Ossabaw (Hamamdzic and Wilensky 2013), and the Rapacz familial hypercholesterolemic (RFH; Granada et al 2009) swine are used for specific advantages they possess in a given area of study. While other mini-swine breeds, such as the Wisconsin Miniature Swine™ at University of Wisconsin–Madison, continue to be developed and refined for specific characteristic advantages, there have been efforts to further miniaturize the mini-swine.…”

Section: Miniature Swinementioning

confidence: 99%

Miniature Swine for Preclinical Modeling of Complexities of Human Disease for Translational Scientific Discovery and Accelerated Development of Therapies and Medical Devices

Schomberg

Téllez²,

Meudt

et al. 2016

Toxicol Pathol

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Noncommunicable diseases, including cardiovascular disease, diabetes, chronic respiratory disease, and cancer, are the leading cause of death in the world. The cost, both monetary and time, of developing therapies to prevent, treat, or manage these diseases has become unsustainable. A contributing factor is inefficient and ineffective preclinical research, in which the animal models utilized do not replicate the complex physiology that influences disease. An ideal preclinical animal model is one that responds similarly to intrinsic and extrinsic influences, providing high translatability and concordance of preclinical findings to humans. The overwhelming genetic, anatomical, physiological, and pathophysiological similarities to humans make miniature swine an ideal model for preclinical studies of human disease. Additionally, recent development of precision gene-editing tools for creation of novel genetic swine models allows the modeling of highly complex pathophysiology and comorbidities. As such, the utilization of swine models in early research allows for the evaluation of novel drug and technology efficacy while encouraging redesign and refinement before committing to clinical testing. This review highlights the appropriateness of the miniature swine for modeling complex physiologic systems, presenting it as a highly translational preclinical platform to validate efficacy and safety of therapies and devices.

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“…The same group measured kidney function in these pigs, and compared it with human kidney function. The glomerular filtration rate of the Banna minipigs was lower than that in humans, whereas renal blood flow and drug metabolism were similar [20].…”

Section: Pre‐clinical Studiesmentioning

confidence: 99%

“…Banna minipigs were studied as potential inbred pig xenograft donors (18)(19)(20). Zhang et al [18] studied hepatic function of these pigs and compared it with humans.…”

Section: Pre-clinical Studiesmentioning

confidence: 99%