Study of some analytical-methodological aspects in nuclear microprobe analysis of soft biological tissues

Lierde, S. Van; Maenhaut, Willy; Reuck, Jacques De; Vis, R.D.

doi:10.1016/0168-583x(95)00443-2

Cited by 13 publications

(1 citation statement)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This encouraging result is even more significant because gelatin standards, commonly used for testing and quantitative analysis of biological materials (Marshall, 1982), are much more sensitive to beam interactions than animal or plant tissues (Marshall, 1980). Mass losses during electron and proton bombardment at room temperature are evident and well documented, especially when high currents are applied (Cholewa et al, 1991;Llabador et al, 1990;Themner, 1991;Themner et al, 1990;Van Lierde, 1995;Zierold, 1988). It is therefore beneficial to perform analyses of fragile materials at low temperature.…”

Section: Limits Of Quantitative Analysismentioning

confidence: 74%

X‐ray microanalysis of biological material in the frozen‐hydrated state by PIXE

Tylko¹,

Mesjasz‐Przybyłowicz²,

Przybyłowicz³

2006

Microscopy Res & Technique

View full text Add to dashboard Cite

Elemental microanalysis of biological material in the frozen-hydrated state using in-vacuum proton induced X-ray emission is described for the first time. For this purpose, a commercially available cryotransfer system was modified and coupled to the experimental chamber of the nuclear microprobe (NMP). The analyzed material was frozen in propane cooled by liquid nitrogen, fractured, carbon coated, and transferred onto the cold stage (100 K) of the nuclear microprobe chamber. Micro-PIXE and simultaneous proton backscattering was performed using a 3 MeV proton beam. Quantitative results were obtained by the standardless method, and tested using 20% gelatin standards. Monitoring of the gas composition inside the system by means of mass spectrometry performed before, during, and after proton bombardment showed good stability of the analyzed material for proton currents not exceeding 150 pA. Average concentrations of light elements (C, N, O, and indirectly H) were also obtained by the proton backscattering technique. No losses of elements measurable by particle-induced X-ray emission (PIXE) during proton irradiation were found during repetitive, short analyses of the same micro areas of gelatin standards. Measurements of thick sections of selected plant and animal material in the frozen-hydrated state-leaf sections of the plant Senecio anomalochrous Hilliard (Asteraceae) and larvae of Chysolina pardalina Fabricius (Chrysomelidae)-showed very good preservation of morphology and elemental distribution. Limits of detection of the order of a few micro g g(-1) were obtained for most elements.

show abstract

Section: Limits Of Quantitative Analysismentioning

confidence: 74%

X‐ray microanalysis of biological material in the frozen‐hydrated state by PIXE

Tylko¹,

Mesjasz‐Przybyłowicz²,

Przybyłowicz³

2006

Microscopy Res & Technique

View full text Add to dashboard Cite

show abstract

Biological applications of the NAC nuclear microprobe

et al. 1999

View full text Add to dashboard Cite

The nuclear microprobe (NMP) is an established analytical instrument for the determination of minor and trace elements. It allows measurements with a spatial resolution of the order of 1 µm and minimum detection limits down to few ppm by weight, with excellent scanning capabilities. The nuclear microprobe of the National Accelerator Centre, South Africa, is being used in a wide number of applications in the biosciences. The complementarity of proton‐induced x‐ray emission and backscattering spectrometry in a wide range of biological applications is shown. The advantages and restrictions of true elemental imaging are also discussed. Copyright © 1999 John Wiley & Sons, Ltd.

show abstract