Study of some parameters which affect xylanase production: Strain selection, enzyme extraction optimization, and influence of drying conditions

Maciel, Giselle Maria; Vandenberghe, Luciana Porto de Souza; Fendrich, Ricardo Cancio; Bianca, Bianca Eli Della; Haminiuk, Charles Windson Isidoro; Soccol, Carlos Ricardo

doi:10.1007/s12257-009-0053-8

Cited by 9 publications

(9 citation statements)

References 23 publications

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“…Sodium chloride was investigated to increase the ionic strength of the buffer and disrupt interactions between protonated groups on proteins and feedstocks. It assisted in the recovery of xylanase from sugarcane bagasse (Maciel et al, 2009). The third component was ethylene glycol, which was selected to disrupt hydrophobic interactions.…”

Section: Discussionmentioning

confidence: 99%

Bioenergy feedstock‐specific enrichment of microbial populations during high‐solids thermophilic deconstruction

Reddy

Allgaier

Singer

et al. 2011

Biotech & Bioengineering

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Thermophilic microbial communities that are active in a high-solids environment offer great potential for the discovery of industrially relevant enzymes that efficiently deconstruct bioenergy feedstocks. In this study, finished green waste compost was used as an inoculum source to enrich microbial communities and associated enzymes that hydrolyze cellulose and hemicellulose during thermophilic high-solids fermentation of the bioenergy feedstocks switchgrass and corn stover. Methods involving the disruption of enzyme and plant cell wall polysaccharide interactions were developed to recover xylanase and endoglucanase activity from deconstructed solids. Xylanase and endoglucanase activity increased by more than a factor of 5, upon four successive enrichments on switchgrass. Overall, the changes for switchgrass were more pronounced than for corn stover; solids reduction between the first and second enrichments increased by a factor of four for switchgrass while solids reduction remained relatively constant for corn stover. Amplicon pyrosequencing analysis of small-subunit ribosomal RNA genes recovered from enriched samples indicated rapid changes in the microbial communities between the first and second enrichment with the simplified communities achieved by the third enrichment. The results demonstrate a successful approach for enrichment of unique microbial communities and enzymes active in a thermophilic high-solids environment.

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Section: Discussionmentioning

confidence: 99%

Bioenergy feedstock‐specific enrichment of microbial populations during high‐solids thermophilic deconstruction

Reddy

Allgaier

Singer

et al. 2011

Biotech & Bioengineering

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“…The enzymes considered include pectinase (Castilho et al, 2000;Singh et al, 1999), xylanase (Heck et al, 2005;Maciel et al, 2009;Pal and Khanum, 2010;Rezaei et al, 2011), lipase (Menoncin et al, 2010), inulinase (Bender Brazilian Journal of Chemical Engineering et al, 2008;Chen et al, 2011), and manganeseperoxidase (Arantes et al, 2011). However, there have been few studies concerning the optimum conditions for cellulase extraction from SSF cultivations.…”

Section: Introductionmentioning

confidence: 99%

Improving the extraction conditions of endoglucanase produced by Aspergillus niger under solid-state fermentation

Pirota

Miotto

Delabona

et al. 2013

Braz. J. Chem. Eng.

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-Production of cellulases under solid-state fermentation (SSF) is a promising technique that can help to reduce costs. Besides optimizing the production process, it is also important to consider enzyme recovery during the extraction step. Here, an experimental design methodology was used to investigate the effects of the operational parameters solid to liquid ratio (1:3, 1:6 and 1:9), stirring rate (80, 120 and 160 rpm), and temperature (10, 22 and 35 °C) on the recovery of endoglucanases produced by Aspergillus niger cultivated under SSF. Statistical analysis revealed that only the solid to liquid ratio had a significant influence on endoglucanase extraction. The highest endoglucanase recovery (35.7 U/g) was achieved using 0.2 mol/L acetate buffer at pH 4.8, together with a solid to liquid ratio of 1:9 and an agitation time of 10 minutes. In sequential extraction experiments, it was shown that most of the enzyme was recovered during the first extraction. The procedure adopted increased the efficiency of endoglucanase extraction by 70%, emphasizing the importance of selection of suitable operational conditions during SSF processes.

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“…Acetate buffer was also found to be the worst extraction buffer for xylanase recovery elsewhere [27]. The negative effect of NaCl in our study suggested that the xylanases may have had binding modules to the surface of the plant polysaccharides which was adversely affected by the presence of ionic compounds.…”

Section: Resultsmentioning

confidence: 68%

“…cellulolyticus . Three chemicals, NaCl, ethylene glycol, and Tween 80 that had been previously used in enzyme extraction studies were examined [ 19 , 22 , 23 ]. NaCl was used to address binding associated with a protonated group, ethylene glycol was examined to interrupt hydrophobic interactions, and Tween 80 was used to address non-specific binding [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Selection of Conditions for Cellulase and Xylanase Extraction from Switchgrass Colonized by Acidothermus cellulolyticus

Rezaei

Joh

Kashima

et al. 2011

Appl Biochem Biotechnol

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Solid-state fermentation has been widely used for enzyme production. However, secreted enzymes often bind to the solid substrate preventing their detection and recovery. A series of screening studies was performed to examine the role of extraction buffer composition including NaCl, ethylene glycol, sodium acetate buffer, and Tween 80, on xylanase and cellulase recovery from switchgrass. Our results indicated that the selection of an extraction buffer is highly dependent on the nature and source of the enzyme being extracted. While a buffer containing 50 mM sodium acetate at pH 5 was found to have a positive effect on the recovery of commercial fungal-derived cellulase and xylanase amended to switchgrass, the same buffer had a significant negative effect on enzyme extraction from solid fermentation samples colonized by the bacterium Acidothermus cellulolyticus. Xylanase activity was more affected by components in the extraction buffers compared to cellulase. This study demonstrated that extraction followed by diafiltration is important for assessing enzyme recovery from solid fermentation samples. Reduction in activity due to compounds present in the switchgrass extracts is reversible when the compounds are removed via diafiltration.

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Study of some parameters which affect xylanase production: Strain selection, enzyme extraction optimization, and influence of drying conditions

Cited by 9 publications

References 23 publications

Bioenergy feedstock‐specific enrichment of microbial populations during high‐solids thermophilic deconstruction

Bioenergy feedstock‐specific enrichment of microbial populations during high‐solids thermophilic deconstruction

Improving the extraction conditions of endoglucanase produced by Aspergillus niger under solid-state fermentation

Selection of Conditions for Cellulase and Xylanase Extraction from Switchgrass Colonized by Acidothermus cellulolyticus

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