Study of the Activity Extracts From Saussurea Controversa and Filipendula Ulmaria in Experimental Osteomyelitis With Three-Phase Scintigraphy

Авдеева, Е. Ю.; Зоркальцев, М. А.; Завадовская, В. Д.; Слизовский, Г. В.; Krasnov, E. A.; Пехенько, Владимир Григорьевич; Степанов, М. Ю.

doi:10.20538/1682-0363-2015-3-5-9

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“…Not only the basic structure of the flavone nucleus and the antioxidant and complexing properties of phenolic groups in the ring have a role in the osteogenic activity of F-SC, but also the number and nature of the bonds of sugar fragments [12,13]. The latter assumption is confirmed by the revealed osteogenic properties of polysaccharides, mainly consisting of sugar monoses and having different types of glycoside chains [16,17,37]. The composition of sugar fragments plays a decisive role in the activity of polysaccharides, and the degree of polymerization of the branch and the degree of branching determine the degree of activity.…”

Section: Discussionmentioning

confidence: 99%

“…We have previously described some active substances isolated from S. controversa extract (native chelidonic acid and its complex with calcium) and their direct in vitro effect on osteogenic differentiation of human multipotent mesenchymal stromal cells (MMSCs) [16] promoting bone regeneration. Osteoprotective in vivo action of S. controversa extract, containing flavonoids and polysaccharides as predominant components, has been recently discovered in a rat model of experimental osteomyelitis caused by Staphylococcus aureus [17]. Flavonoids of S. controversa decreased the intensity of inflammatory processes and stimulated bone regeneration as compared with the antibiotic cefazolin [18].…”

Section: Introductionmentioning

confidence: 99%

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Comparative In Vitro Evaluation of Antibacterial and Osteogenic Activity of Polysaccharide and Flavonoid Fractions Isolated from the leaves of Saussurea controversa

et al. 2019

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The osteogenic, cytotoxic, and antibacterial activities of polysaccharide (PS-SC) and flavonoid (F-SC) fractions isolated from the leaves extract of Saussurea controversa were studied in vitro. F-SC consists of the five quercetin glycosides in the ratio 2:8:10:1:4, which were isolated from the leaves extract of S. controversa and have been characterized previously. PS-SC was first isolated from the leaves extract of S. controversa and has been described. PS-SC consists in 30 compounds is characterized by a high degree of heterogeneity with a heterogeneity index of 19.74. The Mw and Mn of PS-SC were 108.6 and 5.5 kDa, respectively. Structural fragments are represented by galactose, arabinose, xylose, glucose, uronic acids, mannose, and rhamnose in a 10.1:3.3:2.2:2.1:1.7:0.9:0.5 molar ratio. F-SC as compared with PS-SC showed in vitro microbicidal (50 g/L) and better bacteriostatic (6.25 g/L versus 25 g/L of PS-SC) effects against the 24-h growth of Staphylococcus aureus strain 209 P and a 21-day absence of cytotoxicity on human adipose-derived multipotent mesenchymal stromal cells (hAMMSCs). Both fractions (PS-SC > F-SC) at doses of 10–50 mg/L stimulated differentiation of hAMMSCs into secreting osteoblasts accompanied by local mineralization of extracellular matrix. These fractions of S. controversa and especially F-SC, might be promising peroral drugs in the complex treatment of bone fractures and for prophylaxis of their infectious complications.

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