Study of the Annexin A1 and Its Associations with Carcinoembryonic Antigen and Mismatch Repair Proteins in Colorectal Cancer

Ydy, Lenuce Ribeiro Aziz; Santo, Gilmar Ferreira do Espírito; Menezes, Ivana de; Martins, Michelle Santos; Ignotti, Eliane; Damazo, Amílcar Sabino

doi:10.1007/s12029-015-9791-3

Cited by 10 publications

(36 citation statements)

References 22 publications

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“…miR-26a regulated the proliferation and growth of cancer cells by targeting chromodomain helicase DNA binding protein 1 (35), growth regulation by estrogen in breast cancer 1 (35) and β-catenin (36). A number of studies demonstrated that the expression levels of miR-26a decreased in various types of tumor (23,37,38), whereas ANXA1 protein was overexpressed (39)(40)(41). Combined with the results of the present study suggesting that the miR-26a mimic may reduce the expression levels of ANXA1, a negative correlation was identified between miR-26a and ANXA1 in numerous tumors.…”

Section: Discussionsupporting

confidence: 78%

MicroRNA‑26a regulates ANXA1, rather than DAL‑1, in the development of lung cancer

et al. 2018

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Abstract. The aim of the present study was to investigate the expression and role of microRNA-26a (miR-26a) in lung cancer, and to verify whether differentially expressed in adenocarcinoma of the lung (DAL-1) is the target protein of miR-26a. mRNA expression levels of miR-26a and DAL-1 were detected using reverse transcription-quantitative polymerase chain reaction. Protein expression levels of DAL-1 and annexin A1 (ANXA1) were evaluated by western blot analysis. Cell Counting Kit-8, Transwell and wound scratch healing assays were used to characterize the function of miR-26a in lung cancer cells. The association of DAL-1 with miR-26a or ANXA1 was determined by dual-luciferase reporter or two-dimensional gel electrophoresis assays. miR-26a revealed decreased expression levels in lung cancer tissues compared with normal lung tissues, and decreased expression levels in lung cancer cells compared with 16HBE cells. Inhibition of miR-26a promoted lung cancer cell growth, migration and invasion. The DAL-1 protein exhibited downregulated expression levels in lung cancer tissues. DAL-1 was not the direct target gene of miR-26a. The two-dimensional gel electrophoresis assay confirmed that DAL-1 and ANXA1 were associated proteins. Expression levels of the ANXA1 protein were increased following DAL-1 gene silencing. The altered expression level of miR-26a affected the expression of ANXA1, and not of DAL-1. miR-26a demonstrated decreased expression levels in lung cancer cells, and it has an important effect on the biological function of lung cancer cells. However, DAL-1 was not a target gene of miR-26a. As a DAL-1 associated protein, ANXA1 was regulated by miR-26a. IntroductionLung cancer has a high incidence of tumor recurrence and metastasis, and is the most common cause of cancer-associated morality worldwide (1). The overall 5-year survival rate among patients with lung cancer is <15%, and a high rate of metastasis is the primary cause of lung cancer-associated mortality (2). A previous study confirmed that differentially expressed in adenocarcinoma of the lung (DAL-1), a protein that belongs to the membrane-associated cytoskeleton protein 4.1 family, is an efficient suppressor of epithelial-mesenchymal transition (EMT) in lung cancer (3). EMT is a pivotal event in lung cancer metastasis progression (4-6). However, the regulators of DAL-1 in EMT remain unknown.Recently, a novel batch of endogenous small non-coding regulatory RNAs (microRNA's; miRNA's) have received attention in the development of cancer, miRNA's bind complementary sequences in target mRNAs, resulting in selective degradation or selective inhibition of their translation (7). The present study investigated the possible miRNAs of DAL-1 using bioinformatic assays, which included miR-26b, miR-26a, miR-96 and miR-223. Among them, the regulation of DAL-1 by miR-223 has been demonstrated to serve a role in gastric cancer (8). The present study revealed that the gene silencing of DAL-1 induced annexin A1 (ANXA1) protein expression levels to increase in H460 cells...

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Section: Discussionsupporting

confidence: 78%

MicroRNA‑26a regulates ANXA1, rather than DAL‑1, in the development of lung cancer

et al. 2018

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“…Findings presented here are consistent with the data obtained by Su et al (50), reporting annexin A1 overexpressed in colorectal cancer patients with the K-Ras mutation. Similarly, Ydy et al (57) observed higher levels of annexin A1 in colon but not rectum cancer tissue specimens compared with normal margin tissue.…”

Section: Discussionmentioning

confidence: 85%

Proteomics analysis of the matrisome from MC38 experimental mouse liver metastases

Yuzhalin

Lim

Gordon-Weeks

et al. 2019

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…A number of studies have reported aberrant expression levels of ANXAs in various types of cancer, and that ANXAs may function as tumor suppressors or promoters depending on the cancer type. For example, ANXA1 serves a tumor-promoting role in colorectal and lung cancer (9,10), but functions as a tumor suppressor in esophageal and gastric cancer (11). The role of ANXA1 has also been investigated in hepatocellular carcinoma (HCC), but the results are controversial (12).…”

Section: Introductionmentioning

confidence: 99%

Expression levels and prognostic values of annexins in liver cancer

et al. 2019

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Annexins are a superfamily of calcium-dependent phospholipid-binding proteins that are implicated in a wide range of biological processes. The annexin superfamily comprises 13 members in humans (ANXAs), the majority of which are frequently dysregulated in cancer. However, the expression patterns and prognostic values of ANXAs in liver cancer are currently largely unknown. The present study aimed to analyze the expression levels of ANXAs and survival data in patients with liver cancer from the Oncomine, GEPIA, Kaplan-Meier plotter and cBioPortal for Cancer Genomics databases. The results demonstrated that ANXA1, A2, A3, A4 and A5 were upregulated, whereas ANXA10 was downregulated in liver cancer compared with normal liver tissues. The expression of ANXA10 was associated with pathological stage. High expression levels of ANXA2 and A5 were significantly associated with poor overall survival (OS) rate whereas ANXA7 and A10 were associated with increased OS. The prognostic values of ANXAs in liver cancer were determined based on sex and clinical stage, which revealed that ANXA2, A5, A7 and A10 were associated with OS in male, but not in female patients. In addition, the potential biological functions of ANXAs were identified by Gene Ontology functional annotation and Kyoto Encyclopedia of Genes Genomes pathway analysis; the results demonstrated that ANXAs may serve a role in liver cancer through the neuroactive ligand-receptor interaction pathway. In conclusion, the results of the present study suggested that ANXA1, A2, A3, A4, A5 and A10 may be potential therapeutic targets for liver cancer treatment, and that ANXA2, A5, A7 and A10 may be potential prognostic biomarkers of liver cancer.

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Study of the Annexin A1 and Its Associations with Carcinoembryonic Antigen and Mismatch Repair Proteins in Colorectal Cancer

Abstract: The high incidence of ANXA1 positive expression in colorectal cancer and its association with carcinoembryonic antigen levels might indicate the importance of this protein in the colorectal cancer biology.

Cited by 10 publications

References 22 publications

MicroRNA‑26a regulates ANXA1, rather than DAL‑1, in the development of lung cancer

MicroRNA‑26a regulates ANXA1, rather than DAL‑1, in the development of lung cancer

Proteomics analysis of the matrisome from MC38 experimental mouse liver metastases

Expression levels and prognostic values of annexins in liver cancer

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