Study of the anticancer effects of a helium plasma jet combined with four anticancer drugs on 3D bladder tumour spheroids

Abstract

Current studies of cold atmospheric plasma cancer therapy mainly focus on the application of plasma alone and neglect the application of plasma combined with traditional therapeutic techniques. In this paper, the anticancer effects of a plasma jet combined with four clinical anticancer drugs (cisplatin, methotrexate, adriamycin and paclitaxel) on three-dimensional bladder tumour spheroids (T24 cells) were studied. The results show that all four drugs had little impact on the liquidphase oxidative pressure induced by the plasma jet, and anticancer effects from strong to weak were as follows: Plasma jet + paclitaxel, plasma jet + adriamycin, plasma jet + cisplatin, plasma jet and plasma jet + methotrexate. Our research indicates that plasma exhibits selectivity in combination with particular anticancer drugs.

Synergistic effect of cold gas plasma and experimental drug exposure exhibits skin cancer toxicity in vitro and in vivo

“…The small surface contact area enables treatment with high flexibility which is beneficial for skin cancer therapy while minimizing side effects by limiting exposure to the disease area. Additive or synergistic efficacies of cold gas plasmas have been described before, for example, in combination with doxorubicin [52] , [53] , [54] , cisplatin [55] , [56] , [57] , [58] , methotrexate [59] , imiquimod [50] , gemcitabine [60] , [61] , olaparib [62] , ADDA5 [63] , cyclophosphamide [64] , paclitaxel [59] , [65] , [66] , [67] , and temozolomide [68] , [69] , [70] , [71] , [72] , [73] , [74] , [75] across different tumor cell types in vitro and partially also in vivo . Therefore, systemic drug treatment combined with local cold gas plasma treatment may provide a viable strategy to achieve improved anti-tumor efficacies with reduced systemic side effects, provided a local tumor treatment is achievable, as in skin cancer.…”

Foundations of plasmas for medical applications

“…Several synergistic effects of CAP with radiotherapy, PEF, or chemotherapeutics are demonstrated in vitro as well as in animal experiments; see e.g. [303][304][305][306][307][308][309]. Such combination treatments are under investigation because they offer the chance to reduce effective doses of radiation or chemotherapeutics, respectively, and by this means lower side effects of cancer therapy.…”

Two-Phase-Interfaced, Graded-Permittivity Titania Electrical Insulation by Atmospheric Pressure Plasmas

“…Due to the chemical adsorption effect of combined bonds produced by plasma interactions between functional layers and substrates, the long-term stability will be improved significantly. With the help of the active species in the plasma, the interactions of plasma and materials such as the penetration of energetic ions, electrons, and photons into the material, the breaking of bonds, and the recombination of bonds can be in situ tuned. , Thus, the properties of TiO 2 layers can be regulated. The atmospheric pressure plasma deposition without a vacuum system is suitable for a large-scale processing. Especially, an atmospheric pressure plasma jet is convenient for achieving the surface deposition on a 3D substrate The harmful emissions in the plasma deposition processing are negligible .…”

“…The atmospheric pressure plasma deposition without a vacuum system is suitable for a large-scale processing. Especially, an atmospheric pressure plasma jet is convenient for achieving the surface deposition on a 3D substrate …”