Study of the combined effect of electro-activated solutions and heat treatment on the destruction of spores of Clostridium sporogenes and Geobacillus stearothermophilus in model solution and vegetable puree

Liato, Viacheslav; Labrie, Steve; Viel, Catherine; Benali, Marzouk; Aïder, Mohammed

doi:10.1016/j.anaerobe.2015.06.004

Cited by 16 publications

(15 citation statements)

References 52 publications

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“…2009; Luttrell 2012). The anolytes of OSS during the EA treatment acquire important changes in properties which are able to disturb the bacteria homeostasis and act synergistically with heat or other hurdles (Liato et al 2015b). On one hand, the growth of micro-organisms is mostly dependent on the medium acidity; namely the solution pH and its titratable acidity which is a measure of all free and bond hydrogen ions available to maintain a buffering capacity of acidic solution.…”

Section: Discussionmentioning

confidence: 99%

Electro-activation of potassium acetate, potassium citrate and calcium lactate: impact on solution acidity, Redox potential, vibrational properties of Raman spectra and antibacterial activity on E. coli O157:H7 at ambient temperature

2016

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AimsTo study the electro-activation of potassium acetate, potassium citrate and calcium lactate aqueous solutions and to evaluate their antimicrobial effect against E. coli O157:H7 at ambient temperature.Methods and resultsPotassium acetate, potassium citrate and calcium lactate aqueous solutions were electrically excited in the anodic compartment of a four sectional electro-activation reactor. Different properties of the electro-activated solutions were measured such as: solutions acidity (pH and titratable), Redox potential and vibrational properties by Raman spectroscopy. Moreover, the antimicrobial activity of these solutions was evaluated against E. coli O157:H7. The results showed a pH decrease from 7.07 ± 0.08, 7.53 ± 0.12 and 6.18 ± 0.1 down to 2.82 ± 0.1, 2.13 ± 0.09 and 2.26 ± 0.15, after 180 min of electro-activation of potassium acetate, potassium citrate and calcium lactate solution, respectively. These solutions were characterized by high oxidative ORP of +1076 ± 12, +958 ± 11 and +820 ± 14 mV, respectively. Raman scattering analysis of anolytes showed stretching vibrations of the hydrogen bonds with the major changes within the region of 3410–3430 cm−1. These solutions were used against E. coli O157:H7 and the results from antimicrobial assays showed high antibacterial effect with a population reduction of ≥6 log CFU/ml within 5 min of treatment.ConclusionsThis study demonstrated the effectiveness of the electro-activation to confer to aqueous solutions of organic salts of highly reactive properties that differ them from their conjugated commercial acids. The electro-activated solutions demonstrated significant antimicrobial activity against E. coli O157:H7.Significance and impact of studyThis study opens new possibilities to use electro-activated solutions of salts of weak organic acids as food preservatives to develop safe, nutritive and low heat processed foods.

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Section: Discussionmentioning

confidence: 99%

Electro-activation of potassium acetate, potassium citrate and calcium lactate: impact on solution acidity, Redox potential, vibrational properties of Raman spectra and antibacterial activity on E. coli O157:H7 at ambient temperature

2016

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“…Indeed, these radicals could combine with other metastable compounds, resulting in the formation of other organic components (e.g., CH 3 OO • ) that may possibly contribute to the metastable state of such EAS . In a recent work, Liato has reported that the presence of free gaseous oxygen (O 2 ) generated during the electro-activation process obviously contributed to the decrease of EAS pH while the acidification increased in the medium …”

Section: Resultsmentioning

confidence: 99%

“…The cationic exchange membrane (CEM) avoids the transfer of undesired anions from the cathodic to the other compartments, and consequently, anolytes with distinctive properties (pH, p K a , ORP, etc.) could be produced . Then, as shown in Table , the anodic compartment of the reactor was filled with 120 mL of calcium lactate solution or calcium ascorbate, or their equimolar mixture, with a concentration of 10 ppm at time zero of the electro-activation process.…”

Section: Methodsmentioning

confidence: 99%

“…In fact, the electro-activation technique is based on several electrochemical reactions, particularly the electrolysis phenomenon, which is linked to Faraday’s laws. Basically, during the electrolysis process, the electric field induces a migration of anions and cations to the positive (anode) and negative (cathode) electrodes of an electrochemical cell, respectively . Furthermore, the physicochemical reactions that occurred close to the solution–electrode interfaces resulted in two different phenomena called reduction and oxidation, occurring at the cathodic and anodic sections.…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, different compounds are formed, e.g., O 2 , HCl, HClO, ClO – , or NaOH, producing simultaneously some acidic (anolytes) and alkaline (catholytes) solutions in the anodic and cathodic compartments, respectively. In terms of characteristics, it has been reported that anolytes may have acidic pH of about 2–6.5 and positive oxido-reduction potential (ORP) ranging from +200 to +1200 mV under specific conditions. , On the other hand, electro-activated catholytes are rather characterized by alkaline pH, ranging from 7 to 13 and negative ORP between −80 and −900 mV according to the experimental conditions . Moreover, several studies have reported that the significant changes induced by electro-activation in pH and ORP of aqueous solutions may be due to the metastable state of such solutions, which increased their reactivity and rendered them potentially usable as an antimicrobial disinfectant for many fields, namely, food processing and safety, medicine, and biotechnology. , In this context, the factors responsible for the antimicrobial effects of electro-activated solutions (EAS) have been the subject of many investigations in the scientific community.…”

Section: Introductionmentioning

confidence: 99%

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Study of the Electro-Activation Process of Calcium Lactate, Calcium Ascorbate Solutions, and Their Equimolar Mixture: Assessment of Their Physicochemical Properties

et al. 2021

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The aim of this study was to prepare electro-activated solutions (EAS) from calcium lactate, calcium ascorbate, and an equimolar mixture of these two salts to obtain their corresponding acids and to study their physicochemical characteristics, in particular, pH, titratable acidity, pK a, and antioxidant activity. Indeed, the solutions were electro-activated in a reactor comprising three compartments (anodic, central, and cathodic) separated by anionic and cationic exchange membranes, respectively. The electric current intensities used were set at 250, 500, and 750 mA for a maximum period of 30 min. In general, the EAS obtained at 750 mA for 30 min showed the lowest pH (2.16, 2.08, 1.94) and pK a (3.13, 3.07, 2.90) values and the highest titratable acidity (0.107, 0.102, 0.109 mol/L) for calcium lactate, the mixture, and calcium ascorbate, respectively. In addition, the obtained results have demonstrated that the pH, titratable acidity, and pK a of the EAS varied proportionally and significantly (p < 0.001) with the duration of the experiment and the intensity of the electric current applied. To evaluate the migration of calcium (Ca2+) between the central and the cathodic compartments of the reactor, the concentration of Ca2+ was determined especially in the cathodic section by inductively coupled plasma optical emission spectroscopy (ICP-OES). The results showed that the migration of Ca2+ varied proportionally with the electric current intensity. In this context, analysis by Fourier transform infrared (FTIR) spectroscopy, high-performance liquid chromatography (HPLC), and differential scanning calorimetry (DSC) have confirmed the production of lactic acid and ascorbic acid compared to standards. In addition, analysis by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging technique confirmed high antioxidant activities of >90 and >83% for calcium ascorbate and the mixture, respectively, in comparison to the standard ascorbic acid (85%). Overall, this research has clearly demonstrated the eventual potential of electro-activation to produce highly reactive organic acids from their conjugated salts. These EAS can become excellent antimicrobial and sporicidal agents in the food processing industry.

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Human microbiome versus food-borne pathogens: friend or foe

Beeler

Singh

2016

Appl Microbiol Biotechnol

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As food safety advances, there is a great need to maintain, distribute, and provide high-quality food to a much broader consumer base. There is also an ever-growing "arms race" between pathogens and humans as food manufacturers. The human microbiome is a collective organ of microbes that have found community niches while associating with their host and other microorganisms. Humans play an important role in modifying the environment of these organisms through their life choices, especially through individual diet. The composition of an individual's diet influences the digestive system-an ecosystem with the greatest number and largest diversity of organisms currently known. Organisms living on and within food have the potential to be either friends or foes to the consumer. Maintenance of this system can have multiple benefits, but lack of maintenance can lead to a host of chronic and preventable diseases. Overall, this dynamic system is influenced by intense competition from food-borne pathogens, lifestyle, overall diet, and presiding host-associated microbiota.

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Study of the combined effect of electro-activated solutions and heat treatment on the destruction of spores of Clostridium sporogenes and Geobacillus stearothermophilus in model solution and vegetable puree

Cited by 16 publications

References 52 publications

Electro-activation of potassium acetate, potassium citrate and calcium lactate: impact on solution acidity, Redox potential, vibrational properties of Raman spectra and antibacterial activity on E. coli O157:H7 at ambient temperature

Electro-activation of potassium acetate, potassium citrate and calcium lactate: impact on solution acidity, Redox potential, vibrational properties of Raman spectra and antibacterial activity on E. coli O157:H7 at ambient temperature

Study of the Electro-Activation Process of Calcium Lactate, Calcium Ascorbate Solutions, and Their Equimolar Mixture: Assessment of Their Physicochemical Properties

Human microbiome versus food-borne pathogens: friend or foe

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