Study of the Morphological Features of the Cells of the Bacteria Yersinia pestis, Grown at Different Temperatures by Atomic Force Microscopy

Уткин, Д. В.; Булгакова, Е Г; Ерохин, П. С.; Kuznetsov, O. S.; Kuklev, Vasily E.; Осина, Наталья; Microb,

doi:10.18500/1816-9775-2019-19-1-87-93

CBE

2019

DOI: 10.18500/1816-9775-2019-19-1-87-93

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Study of the Morphological Features of the Cells of the Bacteria Yersinia pestis, Grown at Different Temperatures by Atomic Force Microscopy

Д. В. Уткин¹,

Е Г Булгакова²,

П. С. Ерохин³

et al.

Abstract: Ерохин Павел Сергеевич, кандидат физико-математических наук, младший научный сотрудник лаборатории диагностических технологий, Российский научно-исследовательский противочумный институт «Микроб» Роспотребнадзора, Саратов,

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3d-Визуализация Эритроцитов Методами Атомно-Силовой Микроскопии

Трухова¹,

Ахметова²,

Яминский³

2023

NanoRus

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Эритроциты являются одним из любимых объектов исследования в зондовой микроскопии. Во-первых, они легкодоступны, не требуют долгой и сложной пробоподготовки и, что самое главное, изобилуют отличительными особенностями, которые можно применять в клинической диагностике. Когда эритроциты циркулируют в крови, им необходимо проходить через узкие просветы капилляров, намного меньшие, чем размер их собственного поперечного сечения. Упругие свойства мембраны эритроцитов позволяют им проходить по кровяному руслу и доставлять необходимые вещества. Отношение площади поверхности к объему, вязкость цитоплазмы и внутренняя деформируемость мембран влияют на способность эритроцитов трансформироваться и проходить через узкие промежутки. Поэтому морфологические, структурные и физические характеристики клеток крови приобретают все более важное значение при исследовании различных заболеваний или оценке риска их развития.

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3d-Визуализация Эритроцитов Методами Атомно-Силовой Микроскопии

Трухова¹,

Ахметова²,

Яминский³

2023

NanoRus

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Evaluation of azoximer bromide (polyoxidonium) influence on the adhesive properties of the Yersinia pestis EV NIIEG vaccine strain by atomic force microscopy

Щуковская¹,

Goncharova²,

Бугоркова³

et al. 2021

Journal of microbiology, epidemiology and immunobiology

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Aim. To characterize the influence of azoximer bromide (polyoxidonium, PO) in cultivation conditions on the morpho- and nanomechanical cell surface properties of Y. pestis EV NIIEG vaccine strain and its derivatives Y. pestis КМ218 (pYT– , pYV– , pYP– ), Y. pestis КМ216 (pYT– , pYV– , pYP+), Y. pseudotuberculosis, Y. enterocolitica by atomic force microsopy (AFM), as well as on the adhesion of cells Y. pestis EV NIIEG to human collagen type IV.Materials and methods. The measurements were carried out using the Solver P47-PRO probe microscope (NT-MDT, Russia), standard methods of semi-contact AFM and AFM imaging analysis program. The adhesion of Y. pestis EV NIIEG to type IV collagen was determined by the number of cells binding to glass slides covered with human collagen type IV.Results. The introduction of PO in the cultivation environment caused changes in the morphometric parameters of the cells of Y. pestis EV NIIEG vaccine strain and its isogenic derivatives (increase in volume, flatten ingested (S/H), index I (W/H). These changes were accompanied by the transformation of nanomechanical properties of the cell surface (reducing the root mean square, adhesion force), which countenance was associated with the plasmid profile. The lesser decrease of adhesion force in the absence of changes of the index I was observed in cells Y. pseudotuberculosis and Y. enterocolitica with plasmid pYV. In the strain Y. enterocolitica KM383 (pYV– ) PO did not induce significant changes in the indicators studied. The introduction of the PO into the cultivation environment decreased the ability of Y. pestis EV cells to bind to human collagen type IV. Modification by PO the adhesive properties of the vaccine strain Y. pestis EV NIIEG was accompanied by an increase in its immunogenicity

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Study of the Morphological Features of the Cells of the Bacteria Yersinia pestis, Grown at Different Temperatures by Atomic Force Microscopy

Cited by 2 publications

References 0 publications

3d-Визуализация Эритроцитов Методами Атомно-Силовой Микроскопии

3d-Визуализация Эритроцитов Методами Атомно-Силовой Микроскопии

Evaluation of azoximer bromide (polyoxidonium) influence on the adhesive properties of the Yersinia pestis EV NIIEG vaccine strain by atomic force microscopy

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