Study of the relationship between shape and aggregation change in human erythrocytes

Sheremet’ev, Yu. A.; Поповичева, А. Н.; Egorihina, Marpha N; Levin, G. Ya.

doi:10.1134/s0006350913020176

Cited by 5 publications

(2 citation statements)

References 22 publications

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“…RBCs aggregation was investigated using a rheoscope designed by Schmid-Schönbein et al [19] in our modification [11,20]. For investigation of aggregation, RBCs were resuspended in plasma or serum, and the hematocrit was adjusted to 35±0.5%.…”

Section: Assessment Of Rbcs Aggregationmentioning

confidence: 99%

“…Human RBCs aggregation is determined by two types of biophysical and physicochemical factors: suspending phase properties and RBC properties [2,3]. Plasma proteins, such as fibrinogen, α 2 -macroglobulin, and immunoglobulins play a crucial role in RBC aggregation [4][5][6][7][8] Among the cellular factors of RBC aggregation, an important role belongs to the change in surface charge, deformability and shape [3,[9][10][11].…”

Section: Introductionmentioning

confidence: 99%

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Effects of trypsin on aggregation, disaggregation and aggregate morphology of red blood cells in autologous plasma and serum

2021

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We study the influence of trypsin on aggregation, disaggregation, and aggregate morphology of RBCs in autologous plasma and serum. The effect of trypsin on the surface charge of red blood cells and the aggregation of glutaraldehyde fixed cells after treatment with the enzyme was also studied. RBC aggregation was studied by means of an aggregometer and microscopic observations. The results obtained in this study indicate that trypsin treatment increases RBCs aggregation in autologous plasma and serum. The disaggregation of erythrocytes after trypsin treatment considerably decreased in autologous plasma and serum. Increase in the strength of red blood cell aggregates was observed in autologous plasma and serum. The microscopic images of RBCs aggregates indicate the formation of globular (pathologic) structures of aggregates in autologous plasma and serum. Trypsin decrease the surface charge of RBCs. In autologous plasma and serum, the cup shapes of RBCs appear. The control RBCs fixed with glutaraldehyde were not aggregated after their placement in autologous plasma. At the same time, red blood cells pretreated with trypsin and fixed with glutaraldehyde interact with each other in autologous plasma. The physiological significance of glycoproteins of erythrocyte surface for RBCs aggregation was discussed.

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Section: Assessment Of Rbcs Aggregationmentioning

confidence: 99%