Abstract:BackgroundYoung female patients who receive chemotherapy frequently face premature ovarian failure (POF). The therapeutic potential of stem cells in these patients has been explored in stem cells derived from different sources. However, many of these types of stem cells are either difficult to obtain or obtaining them involves invasive procedures. Here, we show that menstrual-derived stem cells (MenSCs) are easy to access and exhibit mesenchymal stem cell-like properties. MenSCs are therefore a novel source of… Show more
“…A week after the injection of busulfan, histological analyses clearly showed that the number of follicles at different stages of development, especially early antral and preovulatory, were reduced, and follicles exhibited poor development and maturity. This result is consistent with that of Wang et al (Wang et al, ). Chemotherapy induced fibrosis formation in ovarian stroma has also been reported, which is similar to the findings of our study (Sun et al, ; Wang et al, ).…”
Section: Discussionsupporting
confidence: 94%
“…GCs of follicles which surround oocytes play a vital role in the development of follicles through secretion of growth factors and hormones (Chavakis, Urbich, & Dimmeler, ; Iorio et al, ). Damage to GCs can thus impair growth, development and survival of oocytes (Wang et al, ).…”
Many studies have reported that human endometrial mesenchymal stem cells (HuMenSCs) are capable of repairing damaged tissues. The aim of the present study was to investigate the effects of HuMenSCs transplantation as a treatment modality in premature ovarian failure (POF) associated with chemotherapy‐induced ovarian damage. HuMenSCs were isolated from menstrual blood samples of five women. After the in vitro culture of HuMenSCs, purity of the cells was assessed by cytometry using CD44, CD90, CD34, and CD45 FITC conjugate antibody. Twenty‐four female Wistar rats were randomly divided into four groups: negative control, positive control, sham, and treatment groups. The rat models of POF used in our study were established by injecting busulfan intraperitoneally into the rats during the first estrus cycle. HuMenSCs were transplanted by injection via the tail vein into the POF‐induced rats. Four weeks after POF induction, ovaries were collected and the levels of Amh, Fst, and Fshr expression in the granulosa cell (GC) layer, as well as plasma estradiol (E2) and progesterone (P4) levels were evaluated. Moreover, migration and localization of DiI‐labeled HuMenSCs were detected, and the labeled cells were found to be localized in GCs layer of immature follicles. In addition to DiI‐labelled HuMenSCs tracking, increased levels of expression of Amh and Fshr and Fst, and the high plasma levels of E2 and P4 confirmed that HuMenSC transplantation had a significant effect on follicle formation and ovulation in the treatment group compared with the negative control (POF) group.
“…A week after the injection of busulfan, histological analyses clearly showed that the number of follicles at different stages of development, especially early antral and preovulatory, were reduced, and follicles exhibited poor development and maturity. This result is consistent with that of Wang et al (Wang et al, ). Chemotherapy induced fibrosis formation in ovarian stroma has also been reported, which is similar to the findings of our study (Sun et al, ; Wang et al, ).…”
Section: Discussionsupporting
confidence: 94%
“…GCs of follicles which surround oocytes play a vital role in the development of follicles through secretion of growth factors and hormones (Chavakis, Urbich, & Dimmeler, ; Iorio et al, ). Damage to GCs can thus impair growth, development and survival of oocytes (Wang et al, ).…”
Many studies have reported that human endometrial mesenchymal stem cells (HuMenSCs) are capable of repairing damaged tissues. The aim of the present study was to investigate the effects of HuMenSCs transplantation as a treatment modality in premature ovarian failure (POF) associated with chemotherapy‐induced ovarian damage. HuMenSCs were isolated from menstrual blood samples of five women. After the in vitro culture of HuMenSCs, purity of the cells was assessed by cytometry using CD44, CD90, CD34, and CD45 FITC conjugate antibody. Twenty‐four female Wistar rats were randomly divided into four groups: negative control, positive control, sham, and treatment groups. The rat models of POF used in our study were established by injecting busulfan intraperitoneally into the rats during the first estrus cycle. HuMenSCs were transplanted by injection via the tail vein into the POF‐induced rats. Four weeks after POF induction, ovaries were collected and the levels of Amh, Fst, and Fshr expression in the granulosa cell (GC) layer, as well as plasma estradiol (E2) and progesterone (P4) levels were evaluated. Moreover, migration and localization of DiI‐labeled HuMenSCs were detected, and the labeled cells were found to be localized in GCs layer of immature follicles. In addition to DiI‐labelled HuMenSCs tracking, increased levels of expression of Amh and Fshr and Fst, and the high plasma levels of E2 and P4 confirmed that HuMenSC transplantation had a significant effect on follicle formation and ovulation in the treatment group compared with the negative control (POF) group.
“…Other members of the lectican PG family do not contain this KS-rich region. [60] The high density of the CS chains in aggrecan provide a high fixed charge density critical to the performance of aggrecan in water imbibition, tissue swelling, and the generation of internal hydrostatic pressure in weight bearing tissues. [6,59] A comparative study of CS and HS in the promotion of 3D chondrogenesis of mesenchymal stem cells (MSCs) showed that CS-hydrogels of low mechanical stiffness provide a scaffold which promotes MSC-based cartilage tissue regeneration.…”
Section: Aggrecan Structure and Functionmentioning
The aim of this study is to review developments in glycosaminoglycan and proteoglycan research relevant to cartilage repair biology and in particular the treatment of osteoarthritis (OA). Glycosaminoglycans decorate a diverse range of extracellular matrix and cell associated proteoglycans conveying structural organization and physico-chemical properties to tissues. They play key roles mediating cellular interactions with bioactive growth factors, cytokines, and morphogenetic proteins, and structural fibrillar collagens, cell interactive and extracellular matrix proteoglycans, and glycoproteins which define tissue function. Proteoglycan degradation detrimentally affects tissue functional properties. Therapeutic strategies have been developed to counter these degenerative changes. Neo-proteoglycans prepared from chondroitin sulfate or hyaluronan and hyaluronan or collagen-binding peptides emulate the interactive, water imbibing, weight bearing, and surface lubricative properties of native proteoglycans. Many neo-proteoglycans outperform native proteoglycans in terms of water imbibition, matrix stabilization, and resistance to proteolytic degradation. The biospecificity of recombinant proteoglycans however, provides precise attachment to native target molecules. Visco-supplements augmented with growth factors/therapeutic cells, hyaluronan, and lubricin (orthobiologicals) have the capacity to lubricate and protect cartilage, control inflammation, and promote cartilage repair and regeneration of early cartilage lesions and may represent a more effective therapeutic approach to the treatment of mild to moderate OA and deserve further study.Similar protective perineural net structures assembled from the lectican proteoglycan family and HA also occur in the CNS/PNS. These so called perineuronal nets are visualized by immunolocalization of MAb 1B5 (+) epitope in rat brain (e) and pericellular 1B5(+) epitope expression by isolated neurons (f). Scale bars 100 µm.
“…The original article [1] contains errors in Figs. 3i, j and 5e whereby the first column of each sub-panel is incorrectly labelled as having used GFP-staining; instead, the images were generated using TUNEL assays.Fig.…”
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