2009
DOI: 10.1007/s11458-009-0033-8
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Study of two-dimensional liquid chromatography with high temperature NPLC and room temperature RPLC

Abstract: To overcome the peak band broadening and to increase the peak capacity and separation efficiency of a two-dimensional liquid chromatographic system, a hightemperature normal phase liquid chromatography (HTNPLC) was used as the first dimension (1 st -D), and a RPLC was used as the second dimension (2 nd -D). The sample was first separated on the 1 st -D CN column and the primary eluent stored in the sampling-loop system alternatively (in HTNPLCÂRPLC mode) or selectively (in HTNPLC/RPLC mode) and was then transf… Show more

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