Study of β-defensin polymorphisms in Valle del Belice dairy sheep

Monteleone, Giuseppina; Calascibetta, Davide; Reina, R.; Portolano, B.

doi:10.4081/ijas.2009.s2.111

Cited by 3 publications

(6 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…No studies have been found that evaluate polymorphisms in the GBD‐1 gene in goats. In sheep, a closer species, Monteleone et al described two SNPs in the β‐defensin 1 gene: at position 1747 of exon 2 and at position 1757 of terminal region 3′. Transitions were A to G and T to C types, respectively.…”

Section: Discussionmentioning

confidence: 94%

See 1 more Smart Citation

Association of polymorphisms of the β‐defensin 1 gene with nematode and protozoan infection traits in goat

Maia

Campêlo

Sarmento

et al. 2019

Parasite Immunology

View full text Add to dashboard Cite

Summary This study's purpose was to identify polymorphisms (SNP) in the goat β‐defensin 1 gene and to associate these SNPs with traits related to nematodean and protozoan infections in Anglo‐Nubian goats from semiarid region of Brazil. A total of 184 animals were used for DNA extraction, PCR and DNA automatic sequencing. The association analyses included the fixed effects of animal age, bloodline and genotype of the SNP marker in the statistical model. The means of genotypes were compared by the Fisher test (P < 0.05). Twelve polymorphism genotypes were found: two in intron 1, seven in exon 2 and three in 3′ untranslated region. The SNPs of exon 2 were responsible for amino acid substitutions in six genetic codes, and the changes in the 25th and 33rd codes affected the protein function. The SNP 1937 was significantly associated with number of protozoan oocysts, whereas SNP 2001 was associated with degree of anaemia (Famacha©). Polymorphism 2046, in turn, showed a significant association with Famacha© degree and number of protozoan oocysts. SNP 2140 associated with maximum EPG of the animal. Results from this study suggest that the β‐defensin 1 gene can be used as a molecular marker for selection of goats regarding the susceptibility to endoparasites infections.

show abstract

Section: Discussionmentioning

confidence: 94%

“…Transitions were A to G and T to C types, respectively. The GBD‐1 gene has been the most polymorphic gene, compared to sheep β‐defensin 1 gene ( SBD‐1 ), as described by Monteleone et al, and to sheep β‐defensin 2 gene ( SBD‐2 ), described by the same authors and Souza et al when analysing a sheep herd from Amazon.…”

Section: Discussionmentioning

confidence: 94%

Association of polymorphisms of the β‐defensin 1 gene with nematode and protozoan infection traits in goat

Maia

Campêlo

Sarmento

et al. 2019

Parasite Immunology

View full text Add to dashboard Cite

show abstract

“…Polymerase chain reaction (PCR) was performed on DNA samples to isolate and amplify a fragment length of 392 bp, comprising the exon II and part of the 3'-UTR of SBD-2. Primers used for PCR were those described by Monteleone et al (2009) Amplifications were carried out in a final volume of 25 µL, containing 1X STR 10X buffer; 3 mM MgCl2; 1.2 mM of each dNTP; 5 pmol primer; 1 U Taq DNA polymerase (500 units, Invitrogen, Life Technology, Carlsbad, CA, USA); and 50-100 ng genomic DNA. The initial desnaturation temperature was 94°C for 5 min, followed by 30 cycles of 94°C for 1 min, 60°C for 45 s, and 72°C for 1 min.…”

Section: Sample and Laboratory Proceedingsmentioning

confidence: 99%

“…In a study evaluating polymorphisms in SBD-2 in Italian sheep, four SNPs were found: one in exon I, and three in exon II (Monteleone et al, 2009). Monteleone et al (2011) conducted a study of Italian sheep and found five SNPs: one in exon I; and four in exon II, some of which were found to cause changes in the SBD-2 amino acid chain.…”

Section: Introductionmentioning

confidence: 99%

Genetic polymorphisms in β-defensin II gene in Amazon sheep from Brazil

et al. 2015

Genet. Mol. Res.

View full text Add to dashboard Cite

The northern region of Brazil produces a large number of sheep, with Pará being the largest sheep breeding state in the region. In the Amazon region, livestock production is a challenge due to the high diversity of pathogens affecting humans and animals. Defensins are antimicrobial peptides acting as a first barrier against micro-organisms and present high variation in different organisms. The objective of this study was to detect polymorphisms in exon II in β-defensin II in Amazon sheep. The gene was amplified by PCR from DNA extracted from 47 sheep blood samples from the Santa Inês breed. Products were sequenced, aligned and analyzed. Three single nucleotide polymorphism (SNP) positions were observed with transition substitutions (A↔G) at positions 1643, 1659, and 1750. The 1643 and 1750 SNPs showed a low variability and significant deviations from Hardy-Weinberg equilibrium (HWE) (P < 0.05) meanwhile the SNP 1659 showed moderate absence of genetic variability and deviation from HWE (P > 0.05). Polymorphisms at 1643 and 1659 were predicted to modify amino acids in the peptide chain (isoleucine to valine and arginine to lysine, respectively) with no effects on protein function. Results from this study suggest that SNPs are important markers for β-defensin II efficiency studies on the immune system of sheep in the Brazilian Amazon.

show abstract

“…SBD-2 contains a total of 64 amino acids. Monteleone et al (2010Monteleone et al ( , 2011 discovered an SNP in the 1659th position of the gene (G to A), which substitutes the 42nd amino-acid arginine for lysine.…”

Section: Introductionmentioning

confidence: 99%

Homology modeling and molecular dynamics of β-defensin II variants in Amazonian sheep

Siqueira¹,

Souza²,

Lima³

et al. 2016

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. β-defensins are capable of creating pores in the bacterial membrane. In this study, we aim to determine the structure of 3 different sheep β-defensin 2 (SBD-2) sequences by molecular modeling. A herd of 47 sheep from the Centre for Ovine and Caprine Research of Pará was selected for this investigation. The AA, AG, and GG alleles were found on β-defensin sequences. We used homology modeling and molecular dynamic simulations to generate 3D models of peptides and they were successfully validated. The proteins are structurally very similar to classic defensins composed of 3 β-sheets and 3 disulfide bonds. Variations in the organization of the tertiary structure and distribution of charged residues were found between AA, AG, and GG alleles. In this study, we were able to characterize and show the structure of 3 SBD-2 gene variants for the first time in Amazonian sheep. Results demonstrated that these variants are similar in structures to classic β-defensins, but contain more positives charges, which may indicate an increase in efficacy.

show abstract

Study of β-defensin polymorphisms in Valle del Belice dairy sheep

Cited by 3 publications

References 12 publications

Association of polymorphisms of the β‐defensin 1 gene with nematode and protozoan infection traits in goat

Association of polymorphisms of the β‐defensin 1 gene with nematode and protozoan infection traits in goat

Genetic polymorphisms in β-defensin II gene in Amazon sheep from Brazil

Homology modeling and molecular dynamics of β-defensin II variants in Amazonian sheep

Contact Info

Product

Resources

About