Study on an antimicrobial protein produced by Paenibacillus polymyxa JSa-9 isolated from soil

Abstract

Paenibacillus polymyxa strain JSa-9, a soil isolate showed in vitro inhibitory effects on several pathogens. A protein of about 71.9 kDa, isolated from a culture of JSa-9, exhibited broad-spectrum antibacterial and antifungal activities. The purification procedure consisted of ion-exchange chromatography on DEAE-Sephacel and Sephadex G-100 column chromatography. The purified protein was characterized to be a glycoprotein, which contained 53% of amino acids and 28% of carbohydrate. Its N-terminal sequence was determined as KCATIPVVIKHL and the amino acid composition showed no significant homology with any known antagonistic proteins from P. polymyxa. Because of the excellent antimicrobial activity, this protein may be a good prospect for food preservation and plant disease control.

Biocontrol of Fusarium Crown and Root Rot and Promotion of Growth of Tomato byPaenibacillusStrains Isolated from Soil

“…Recently, numerous studies have described advantages of using endospore-forming Paenibacillus spp. in agriculture, including plant growth promotion and the suppression of plant diseases [ 5 , 6 ]. However, Paenibacillus spp.…”

“…Among these, Paenibacillus populations were found to be capable of suppressing several plant diseases and promoting plant growth [5, 6]. Paenibacillus populations synthesize the plant hormones auxin and cytokinin, solubilize soil phosphorus, and produce biological control substances like siderophores and antibiotics [7].…”

“…Among the myriads of bacteria thriving in the soil, some spore-forming PGPR, in particular gram-positive bacilli and streptomycetes, have attracted special attention due to their advantages over non-spore formers in product formulation and stable maintenance in soil [ 4 ]. Among these, Paenibacillus populations were found to be capable of suppressing several plant diseases and promoting plant growth [ 5 , 6 ]. Paenibacillus populations synthesize the plant hormones auxin and cytokinin, solubilize soil phosphorus, and produce biological control substances like siderophores and antibiotics [ 7 ].…”

Biocombinatorial Synthesis of Novel Lipopeptides by COM Domain-Mediated Reprogramming of the Plipastatin NRPS Complex

“…Serial dilutions of antimicrobial compounds were prepared to obtain final concentrations of 1000, 500, 250, 125, 62.5, 31.25, 15.63, and 7.81 μg/ml in LB or PDA medium. The ability of cyclic products strains to inhibit the growth of various indicator organisms was determined qualitatively by agar-well diffusion method (Deng et al, 2011a). Plates were incubated for up to 24 (bacteria) and 48 h (fungi) before the antimicrobial activities were determined (Oh et al, 2011).…”

Medium optimization to analyze the protein composition of Bacillus pumilus HR10 antagonizing Sphaeropsis sapinea

“…Liu et al ( 2007 ) used a DEAE-Sepharose Fast Flow weak ion exchange column to chromatograph the crude protein secreted by B. subtilis B-916 strain, and after obtaining an active elution peak, the protein spectrum identified a novel antibacterial protein, Bacisubin, with a molecular weight of 41.900 kDa. The purification procedure of ion-exchange chromatography on DEAE-Sephacel and Sephadex G-100 column chromatography isolated a glycoprotein with a molecular weight of approximately 71.9 kDa from Paenibacillus polymyxa Jsa-9 fermentation broth, which exhibited broad-spectrum antibacterial and antifungal activities (Deng et al 2011 ).…”