Study on Anti‐Inflammatory Effect and Major Anti‐Inflammatory Components of PSORI‐CM02 by Zebrafish Model

Abstract

PSORI-CM02 is an optimization formula of PSORI-CM01, which is a clinical herbal formula for the treatment for psoriasis in the Guangdong Provincial Hospital of Chinese Medicine. Previous research indicates that it plays a critical role in anti-inflammation and immunoregulation. Rhizoma smilacis glabrae (RSG) is one herbal medicine of PSORI-CM02, whose effective anti-inflammatory component is astilbin. This study aims to test the anti-inflammatory and immunoregulation effects of astilbin as well as RSG in PSORI-CM02, and we, respectively, used the CuSO4-induced neutrophil-specific transgenic zebrafish model Tg(mpx: EGFP) and the melanin allele mutated Albino strain zebrafish model to visualize the effects of neutrophil recruitment and macrophage phagocytosis. Our data indicated that both PSORI-CM02 and astilbin had anti-inflammatory effects, leading to a reduction in the recruitment of neutrophils and promotion in macrophage phagocytosis. Nevertheless, the negative liquor of Rhizoma smilacis glabrae (PSORI-CM02 without RSG) also had anti-inflammatory and promoting macrophage phagocytosis effects. The results revealed the formula excluding RSG also had anti-inflammatory and immunoregulation effects, which demonstrated that RSG was not the major anti-inflammatory herbal medicine in PSORI-CM02. Similarly, astilbin was not the major anti-inflammatory active ingredient in the formula. The anti-inflammatory and the promotion of macrophage phagocytosis effect of PSORI-CM02 in vivo zebrafish were the results of multiple component interaction, which was the common characteristic of the Chinese medicine compound.

Kaempferol-3-O-Glucuronide Ameliorates Non-Alcoholic Steatohepatitis in High-Cholesterol-Diet-Induced Larval Zebrafish and HepG2 Cell Models via Regulating Oxidation Stress

“…Tg ( mpx : EGFP) zebrafish can directly reflect the level of neutrophils in vivo experiments, and were used to investigate anti-inflammatory compounds in previous studies [ 25 ]. Recently, anti-inflammatory components of PSORI-CMo2 were selected by Tg ( mpx : EGFP) in Yuan’s research [ 32 ]. In the present study, a significant anti-inflammatory effect of K3O (40 μM) was observed in HCD-induced zebrafish.…”

Molecular Networking-Driven Discovery of Antibacterial Perinadines, New Tetracyclic Alkaloids from the Marine Sponge-Derived Fungus Aspergillus sp.

“…This in vivo anti-inflammatory experiment used the same zebra fish model as that of the previously described protocol. 15 The method was performed by monitoring the number of neutrophils in zebra fish, which are highly similar to mammals in morphology and physiology, and its embryo’s optical transparency makes it possible for one to noninvasively obtain dynamic imaging of the inflammation in vivo . 16 Indometacin was used as a positive control.…”