Study on antibacterial and flavonoid content of ethanolic extract of Punica granatum (pomegranate) peel

Jaisinghani, Renu Narendra; Makhwana, Shweta; Kanojia, Ankit

doi:10.4081/mr.2018.7480

Cited by 17 publications

(10 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, this was contrary to the findings of Nobakht et al [33] who reported highest activity of flavonoids on P. aerugionsa and no activity on S. aureus with mean and standard deviation zone of inhibition of 24.7±2.9 mm and 0.0±0.0 respectively. Jaisinghani et al [34] also reported higher antibacterial activity flavonoids in S. aureus than P. aeruginosa at 100-25 mg/mL concentration compared to 500 mg/mL of extract in this study. It was concluded by Singh et al [30] that flavonoid studied had higher activity against gram positive bacteria than gram negative in which P. aeruginosa had zone of inhibition of 16.33 mm and 18.33 mm in S. aureus.…”

Section: Antibacterial Susceptibility Study Of Crude Flavonoid Extractsupporting

confidence: 74%

Anti-bacterial Activity of Crude Flavonoid Fraction from Bidens pilosa Leaves against Selected Chronic Wound Bacterial Pathogens

Nakibuule¹,

Ntulume²,

Mwandah³

et al. 2019

JOCAMR

View full text Add to dashboard Cite

Aims: Bidens pilosa is an extraordinary source of phytochemicals particularly flavonoids especially in leaves which have been attributed in various studies due to its antibacterial properties. The present study aimed at addressing bio-burden of chronic wound through proving a possible source of new antimicrobial product for wound healing. Methodology: Solvent-solvent extraction method was used to isolate crude flavonoid fraction from leaves of B. pilosa using ether, chloroform, ethylacetate and methanol (1:1:1). Thin-layer chromatography was used to identify crude flavonoid fraction using methanol/n-hexane (1:2: v/v) as mobile phase solvents. Agar well diffusion method was used to determine anti-bacterial activity of crude flavonoid against bacterial pathogens: Susceptible Pseudomonas aeruginosa ATCC®27853™, resistant Pseudomonas aeruginosa susceptible Staphylococcus aureus ATCC®25923™, methicillin resistant Staphylococcus aureus, Streptococcus pneumoniae and methicillin resistant Staphylococcus epidermidis. Minimum inhibitory concentration (MIC) and Minimum bactericidal contrition (MBC) were also determined using broth dilution and culture methods. Results: Thin-layer chromatographic profiling revealed an identity of three different spots with flavonoids from B. pilosa leaves showing three bands with Rf values 0.51, 0.60 and 0.63. The mean and standard deviation zone of inhibition of crude flavonoids ranged from 11.50±0.50 mm to 17.50±1.50 mm. The mean and standard deviation of positive controls (Ciproflaxacin, Co-Amoxiclay and Voncomycin) ranged from 0.00±0.00 to 22.50±0.50 mm. MIC and MBC of crude flavonoids ranged from 12.5-25.0 mg/mL and 50 to 200 mg/mL respectively. The flavonoid fraction was more effective against gram positive bacteria than on gram negative bacteria and it exhibited bactericidal effect on Methicillin resistant Staphylococcus aureus, resistant P. aureginosa, sensitive P.aureginosa and S. pneumonia. Conclusion: B. pilosa leaves could be a potential source for future drug development from flavonoid to address the issue of need for new antibiotics due to alarming burden of antimicrobial resistance in last resort antibiotics.

show abstract

Section: Antibacterial Susceptibility Study Of Crude Flavonoid Extractsupporting

confidence: 74%

Anti-bacterial Activity of Crude Flavonoid Fraction from Bidens pilosa Leaves against Selected Chronic Wound Bacterial Pathogens

Nakibuule¹,

Ntulume²,

Mwandah³

et al. 2019

JOCAMR

View full text Add to dashboard Cite

show abstract

“…aureus and it was more effective against gram positive bacteria than gram negative. In the study carried by Jaisinghani et al, 2018. The inhibition zone was greater against S. Typhimurium than Staph.…”

Section: Discussionmentioning

confidence: 93%

Portrayal of Punica granatum L. peel extract through High Performance Liquid Chromatography and antimicrobial activity evaluation

Mahmood¹,

Ashraf

Ali³

et al. 2023

Braz. J. Biol.

View full text Add to dashboard Cite

Increasing trend in antimicrobial resistance and failure of chemically synthesized antibiotics lead to discover alternative methods for the treatment of bacterial infections. Various medicinal plants are in use traditionally and their active compounds can be further applied for treatment of bacterial diseases. This study was designed to determine the antibacterial activity of Punica granatum (P. granatum L.) (pomegranate) peel extract against Enterobacteriaceae [Escherichia coli (E. coli), Salmonella Typhimurium (S. Typhimurium) and Shigella Dysenteriae (S. Dysenteriae)] and gram-positive bacterium [Staphylococcus aureus (Staph aureus)]. Methanolic extract of P. granatum L. peel was prepared by Soxhlet apparatus method. Total flavonoid and phenolic contents from the extract were determined by High Performance Liquid Chromatography (HPLC). The antibacterial activity of P. granatum L. peel extract was evaluated through agar well diffusion method. HPLC showed the range of phenolics (gallic acid, caffeic acid, benzoic acid, cinnamic acid) and flavonoid compounds. The chemical structures of flavonoid and phenolics found in the methanolic extract of P. granatum L. peel have been reported for the first time. The methanolic peel extract (50 ul) of yellow P. granatum L. showed 26, 10, 10 and 9mm zones of inhibition (ZOI) against S. aureus, S. Typhimurium, S. Dysenteriae and E. coli, respectively. The methanolic extract of red P. granatum L. (100 ul) showed 27, 8, 12 and 15 mm ZOI against Staph. aureus, S. Typhimurium, S. Dysenteriae and E. coli, respectively. Highest ZOI was observed against Staph. aureus. Many of the bacteria studied in the present work may cause serious gastrointestinal infections, which can lead to hemorrhagic diarrhea in children. These infections can be life-threatening to young children and the elderly. There is an incentive to find alternative control measures, such as plant and herbal extracts, especially in lesser-developed countries where traditional antibiotics may not be readily available.

show abstract

“…In other studies, researchers found that PPE had an equivalent strength against S. aureus , Salmonella sp., E. coli , etc. [ 22 ]. The antibacterial activity of PPE was associated with its effect on impairing the membrane integrity of bacteria.…”

Section: Resultsmentioning

confidence: 99%

Compositions and Biological Activities of Pomegranate Peel Polyphenols Extracted by Different Solvents

Feng

Lin

et al. 2022

Molecules

View full text Add to dashboard Cite

Pomegranate peel extract (PPE), which is abundant in polyphenols, holds immerse prospects for the treatment of airway infection. In this study, water and ethanol of 30%, 50%, and 80% were used to prepare PPE. A total of 18 phenols belonging to 8 categories of polyphenols were identified in PPE by HPLC-MS/MS. The PPE from the four extraction solvents possessed different antioxidant, antibacterial, and anti-inflammatory activities. Principal component analysis revealed that though total flavonoids (TFs), total polyphenols (TPs), and total tannins (TTs) were responsible for the reducing power of PPE, only TFs contributed to the effect of PPE in inhibiting lipid membrane peroxidation. TPs, TTs, and punicalagin were positively correlated with the antibacterial strength against S. aureus while TTs alone contributed to the inhibition of methicillin-resistant S. aureus, implying the crucial role of TT in suppressing bacteria. Meanwhile, TTs was associated with the prevention of IL-6 release. The PPE with higher contents of TPs, TTs, and punicalagin had a weaker capacity to decrease nitric oxide secretion. PPE of 30% ethanol gained the highest integrated score due to its stronger antioxidant, antibacterial, and anti-inflammatory activities. It is a suitable candidate for the therapy of respiratory tract infection.

show abstract

Study on antibacterial and flavonoid content of ethanolic extract of Punica granatum (pomegranate) peel

Cited by 17 publications

References 10 publications

Anti-bacterial Activity of Crude Flavonoid Fraction from Bidens pilosa Leaves against Selected Chronic Wound Bacterial Pathogens

Anti-bacterial Activity of Crude Flavonoid Fraction from Bidens pilosa Leaves against Selected Chronic Wound Bacterial Pathogens

Portrayal of Punica granatum L. peel extract through High Performance Liquid Chromatography and antimicrobial activity evaluation

Compositions and Biological Activities of Pomegranate Peel Polyphenols Extracted by Different Solvents

Contact Info

Product

Resources

About