Study on biodegradation kinetics of di-2-ethylhexyl phthalate by newly isolated halotolerant Ochrobactrum anthropi strain L1-W

Abstract

Objective: Di-2-ethylhexyl phthalate (DEHP) pollution is one of the major environmental concerns all over the world. This research aimed at studying the biodegradation kinetics of DEHP by a newly isolated bacterial strain. Water and sediment samples were collected from Wuhan South Lake and potent bacterial isolates were screened for DEHP degradation, characterized by biochemical, physiological, morphological and 16S rDNA gene sequencing, and optimized under suitable pH, temperature, NaCl and DEHP concentrations. DEHP and its metabolites were quantified by High Performance Liquid Chromatography and their degradation kinetics were studied. Results: The newly isolated bacterium was identified as Ochrobactrum anthropi strain L1-W with 99.63% similarity to Ochrobactrum anthropi ATCC 49188. It was capable of utilizing DEHP as the carbon source. The optimum growth temperature, pH, DEHP and NaCl concentration for the strain L1-W were 30 °C, 6, 400 mg/L and 10 g/L respectively. Strain L1-W was capable of degrading almost all (98.7%) of DEHP when the initial concentration was 200 mg/L within a period of 72 h. Besides, it was also found capable of degrading five other phthalates, thus making it a possible candidate for bioremediation of phthalates in the environmental settings.

Detection of Bacillus Species with Arsenic Resistance and Plant Growth Promoting Efficacy from Agricultural Soils of Nepal

“…Genomic DNA of isolated Bacillus species was extracted by phenol-chloroform assay, and DNA amplification of the 16S rRNA gene was performed by the primer sets: 8F (5′-AGAGTTTGATCCCTCAG-3′) and 1492R (5′-GGTTACCTTGTTACGACTT-3′) [ 22 , 34 , 37 , 38 ]. PCR amplification conditions were as follows: 30 cycles of denaturation at 98°C for 10 seconds and annealing at 55°C for 5 seconds with final elongation at 72°C for 1 min.…”

Screening and characterization of potent poly glutamic acid producing Bacillus sp. isolated from Kinema, water and soil samples

“…DNA denaturation (30 cycles) at 98 °C for 10 seconds, primer annealing at 55 °C for 5 s, and elongation at 72 °C for 1 min were performed during the PCR. Amplification of the 16S rRNA gene was performed using the following universal primer sets: 8F (5′-AGAGTTTGATCCCTCAG-3′) and 1492R (5′-GGTTACCTTGTTACGACTT-3′) [ 41 , 42 , 43 ]. By loading 7 μL of each PCR product with 2 μL of loading dye into the wells of 0.8% agarose gel containing 2 μL/mL EtBr (ethidium bromide), the amplified DNA fragments were separated using gel electrophoresis.…”

Extremely oligotrophic and complex-carbon-degrading microaerobic bacteria from Arabian Sea oxygen minimum zone sediments

“…For instance, Brevibacterium species (Bhadra et al 2008;Chen et al 2016;Pie et al 2020) have been retrieved from deep subsurfaces of the Pacific and Indian Oceans. Strains of Brucella have been retrieved from the deep subsurface of the Atlantic Ocean (Odumosu et al 2020), while halotolerant strains of the genus have also been unearthed from lacustrine sediments (Nshimiyimana et al 2020). Gordonia species have been retrieved from the sediments of marine tidal flats (Kim et al 2019) and mangrove vegetations (Sangkanu et al 2019).…”