Study on Catalpol Content in &lt;i&gt;Rehmannia glutinosa&lt;/i&gt; Root, an Important Ingredient in Kampo Prescriptions

Huang, Yuting; Ando, Hirokazu; Tsujino, Mai; Yoshihara, Kazuki; Zhang, Li; Sasaki, Yohei

doi:10.1248/bpb.b21-01095

Cited by 5 publications

(2 citation statements)

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“…Therefore, CMCs can be conveniently used for in vitro production of specific metabolites. Catalpol is one of the criteria for evaluating the medicinal effects of R. glutinosa [ 22 ]. In the current study, a higher content of catalpol and its derivative metabolites was recorded in CMCs (Figs.…”

Section: Discussionmentioning

confidence: 99%

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Omics analyses of Rehmannia glutinosa dedifferentiated and cambial meristematic cells reveal mechanisms of catalpol and indole alkaloid biosynthesis

Zhou,

Li,

Lin

et al. 2023

BMC Plant Biol

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Background Rehmannia glutinosa is a rich source of terpenoids with a high medicinal reputation. The present study compared dedifferentiated cells (DDCs) and cambial meristematic cells (CMCs) cell cultures of R. glutinosa for terpenoid (catalpol) and indole alkaloid (IA) biosynthesis. In this regard, we used widely targeted metabolomics and transcriptome sequencing approaches together with the comparison of cell morphology, cell death (%), and catalpol production at different time points. Results We were able to identify CMCs based on their morphology and hypersensitivity to zeocin. CMCs showed higher dry weight content and better catalpol production compared to DDCs. The metabolome analysis revealed higher concentrations of IA, terpenoids, and catalpol in CMCs compared to DDCs. The transcriptome sequencing analysis showed that a total of 27,201 genes enriched in 139 pathways were differentially expressed. The higher catalpol concentration in CMCs is related to the expression changes in genes involved in acetyl-CoA and geranyl-PP biosynthesis, which are precursors for monoterpenoid biosynthesis. Moreover, the expressions of the four primary genes involved in monoterpenoid biosynthesis (NMD, CYP76A26, UGT6, and CYP76F14), along with a squalene monooxygenase, exhibit a strong association with the distinct catalpol biosynthesis. Contrarily, expression changes in AADC, STR, and RBG genes were consistent with the IA biosynthesis. Finally, we discussed the phytohormone signaling and transcription factors in relation to observed changes in metabolome. Conclusions Overall, our study provides novel data for improving the catalpol and IA biosynthesis in R. glutinosa.

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Section: Discussionmentioning

confidence: 99%

“…The catalpol content was determined after 9, 12, 15, 18, and 21 h of cell culture growth as reported earlier [ 22 ].…”

Section: Methodsmentioning

confidence: 99%