Study on cellulase (Β-1,4-endoglucanase) activity of gut bacteria of Sitophilus oryzae in cellulosic waste biodegradation

Prasad, Rajesh Kumar; Chatterjee, Soumya; Mazumder, Pranab Behari; Sharma, Sonika; Datta, Sibnarayan; Vairale, Mohan G.; Dwivedi, Sanjai K.

doi:10.1016/j.biteb.2019.100274

Cited by 8 publications

(2 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To achieve this goal, lter paper collected after bacterial treatment and from control experiments after 14 days was separately mixed with 1000 mg of spectroscopy-grade potassium bromide (KBr) in an agate mortar and subsequently pressed into discs. The infrared (IR) spectra were recorded on an FTIR spectrometer in transmission band mode, ranging from 400 to 4000 cm − 1 [41,28]. SEM and FTIR analyses were also conducted at the instruments available at the Jawaharlal Nehru Centre for Advanced Scienti c Research (JNCASR) in Bengaluru.…”

Section: Sem and Ftir Analysis Of The Hydrolysed Substratementioning

confidence: 99%

Prospecting Cellulolytic Bacteria From White Grubs (Holotrichia serrata (F.) and Leucopholis coneophora Burmeister) Native to Karnataka Region

KR,

Ramesh

et al. 2024

Preprint

View full text Add to dashboard Cite

Background Insecta, the largest class within Arthropoda, hosts complex symbiotic relationships with microorganisms, offering significant potential for various biotechnological applications. The gut microbiota of insects, comprising a diverse array of bacteria, plays crucial roles in food digestion, nutrient provisioning, and enzyme synthesis, particularly relevant for lignocellulosic biomass breakdown and bioremediation. White grubs that are significant pests in Karnataka region, such Holotrichia serrata and Leucopholis coneophora, have gut microbiota that are rich in cellulolytic bacteria, which significantly aid in the breakdown of cellulose. Lignocellulosic biomass, derived from agricultural and forestry waste, presents opportunities for sustainable biofuel production and waste management. Results Seventeen cellulolytic bacterial isolates were obtained from the fermentation chambers of white grubs, displaying diverse morphological characteristics and predominantly belonging to Firmicutes and γ-proteobacteria. Bacillus, Enterobacter and Klebsiella species were prevalent among the isolates. Evaluation of cellulolytic activity revealed notable capabilities, with Bacillus toyonensis strain LC3B1 exhibiting remarkable cellulolytic index. Substrate degradation assays demonstrated LC3B1 proficiency in decomposing various agricultural residues, highlighting its potential in biomass conversion. Structural analyses using FTIR and SEM techniques provided insights into cellulose breakdown mechanisms by these isolates. Conclusion The study underscores the diverse cellulolytic capabilities harbored within the gut bacteria of white grubs, offering promising avenues for biotechnological applications in lignocellulosic biomass degradation and biofuel production. The ubiquity of cellulase-producing strains from families such as Bacillaceae, Enterobacteriaceae, and Pseudomonadaceae highlights their vital role in the degradation of lignocellulosic biomass, having noteworthy consequences for the utilisation of biodegradation in recycling and environmentally friendly waste disposal.

show abstract

Section: Sem and Ftir Analysis Of The Hydrolysed Substratementioning

confidence: 99%

Prospecting Cellulolytic Bacteria From White Grubs (Holotrichia serrata (F.) and Leucopholis coneophora Burmeister) Native to Karnataka Region

KR,

Ramesh

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Bacteria from the phyla Actinobacteria [ 145 , 146 ], Proteobacteria [ 147 ], and Bacillota also produce many cellulases [ 148 ]. Cellulases isolated from bacteria include β-1,4-endoglucanase from Cellulomonas telluris [ 149 ], Bacillus and γ-Protobacteria [ 133 ]; Cellulose 1,4-beta-cellobiosidase from Sporocytophaga myxococcoides and Cohnella sp [ 150 ]; and xylanase from Pseudomonas mohnii [ 151 ].…”

Section: Lignocellulosic Biorefineries and The Roles Of Lignocellulol...mentioning

confidence: 99%

Engineered yeasts and lignocellulosic biomaterials: shaping a new dimension for biorefinery and global bioeconomy

Asemoloye,

Bello,

Oladoye

et al. 2023

Bioengineered

View full text Add to dashboard Cite

The next milestone of synthetic biology research relies on the development of customized microbes for specific industrial purposes. Metabolic pathways of an organism, for example, depict its chemical repertoire and its genetic makeup. If genes controlling such pathways can be identified, scientists can decide to enhance or rewrite them for different purposes depending on the organism and the desired metabolites. The lignocellulosic biorefinery has achieved good progress over the past few years with potential impact on global bioeconomy. This principle aims to produce different bio-based products like biochemical(s) or biofuel(s) from plant biomass under microbial actions. Meanwhile, yeasts have proven very useful for different biotechnological applications. Hence, their potentials in genetic/metabolic engineering can be fully explored for lignocellulosic biorefineries. For instance, the secretion of enzymes above the natural limit (aided by genetic engineering) would speed-up the down-line processes in lignocellulosic biorefineries and the cost. Thus, the next milestone would greatly require the development of synthetic yeasts with much more efficient metabolic capacities to achieve basic requirements for particular biorefinery. This review gave comprehensive overview of lignocellulosic biomaterials and their importance in bioeconomy. Many researchers have demonstrated the engineering of several ligninolytic enzymes in heterologous yeast hosts. However, there are still many factors needing to be well understood like the secretion time, titter value, thermal stability, pH tolerance, and reactivity of the recombinant enzymes. Here, we give a detailed account of the potentials of engineered yeasts being discussed, as well as the constraints associated with their development and applications.

show abstract

Insect gut bacteria: a promising tool for enhanced biogas production

Show

Banerjee

et al. 2022

Rev Environ Sci Biotechnol

View full text Add to dashboard Cite

Study on cellulase (Β-1,4-endoglucanase) activity of gut bacteria of Sitophilus oryzae in cellulosic waste biodegradation

Cited by 8 publications

References 30 publications

Prospecting Cellulolytic Bacteria From White Grubs (Holotrichia serrata (F.) and Leucopholis coneophora Burmeister) Native to Karnataka Region

Prospecting Cellulolytic Bacteria From White Grubs (Holotrichia serrata (F.) and Leucopholis coneophora Burmeister) Native to Karnataka Region

Engineered yeasts and lignocellulosic biomaterials: shaping a new dimension for biorefinery and global bioeconomy

Insect gut bacteria: a promising tool for enhanced biogas production

Contact Info

Product

Resources

About