Abstract:In this study, we composited mineralized collagen and magnesium-calcium alloy by freeze-drying, followed by dip-coating PMMA bone cement to enhance the composite of mineralized collagen and magnesium-calcium alloy. In vitro degradation test was performed to observe the pH and
weight loss of the material. The contact angle test was used to detect the hydrophilicity of the material. Subsequently, MC3T3-E1 were used to assess cell biocompatibility In vitro Show more
“…Guo et al found that the composites of CS and Mg alloy could promote the high expression of ALP activity in MG63 cells. 39 In our previous work, polymethyl methacrylate (PMMA) was used to prepare PMMA/nHAC/Mg-Ca composites and found the PMMA/nHAC/Mg-Ca composites can promote the osteogenic differentiation and mineralization of the MC3T3-E1 cells, 52 which is consistent with our results.…”
Section: Comparison Of the Biocompatibility Of The Cs-nhac/ Mg-ca Com...supporting
Magnesium (Mg) alloy with good mechanical properties and biodegradability is considered as one of the ideal bone repair materials. However, the rapid corrosion of Mg-based metals can pose harm to the function of an implant in clinical applications. In this study, micro-arc oxidation coating was prepared on the surface of the Mg–Ca matrix, then the chitosan and mineralized collagen (nano-hydroxyapatite/collagen; nHAC) were immobilized on the surface of the MAO/Mg–Ca matrix to construct the CS-nHAC/Mg–Ca composites of different component proportions (the ratio of CS to nHAC is 2:1, 1:1, and 1:2, respectively). The corrosion resistance, osteogenic activity, and angiogenic ability were extensively investigated. The results indicated that the CS-nHAC reinforcement materials can improve the corrosion resistance of the Mg matrix significantly and promote the proliferation and adhesion of mouse embryo osteoblast precursor cells (MC3T3-E1) and human umbilical vein endothelial cells (HUVECs). In addition, the CS-nHAC/Mg–Ca composites can not only promote the alkaline phosphatase (ALP) activity and extracellular matrix mineralization of MC3T3-E1 cells but also enhance the migration motility and vascular endothelial growth factor (VEGF) expression of HUVECs. Meanwhile, the 2CS-1nHAC/Mg–Ca composite exhibited the optimum function characteristics compared with other samples. Therefore, considering the improvement of corrosion resistance and biocompatibility, the CS-nHAC/Mg–Ca composites are expected to be a promising orthopedic implant.
“…Guo et al found that the composites of CS and Mg alloy could promote the high expression of ALP activity in MG63 cells. 39 In our previous work, polymethyl methacrylate (PMMA) was used to prepare PMMA/nHAC/Mg-Ca composites and found the PMMA/nHAC/Mg-Ca composites can promote the osteogenic differentiation and mineralization of the MC3T3-E1 cells, 52 which is consistent with our results.…”
Section: Comparison Of the Biocompatibility Of The Cs-nhac/ Mg-ca Com...supporting
Magnesium (Mg) alloy with good mechanical properties and biodegradability is considered as one of the ideal bone repair materials. However, the rapid corrosion of Mg-based metals can pose harm to the function of an implant in clinical applications. In this study, micro-arc oxidation coating was prepared on the surface of the Mg–Ca matrix, then the chitosan and mineralized collagen (nano-hydroxyapatite/collagen; nHAC) were immobilized on the surface of the MAO/Mg–Ca matrix to construct the CS-nHAC/Mg–Ca composites of different component proportions (the ratio of CS to nHAC is 2:1, 1:1, and 1:2, respectively). The corrosion resistance, osteogenic activity, and angiogenic ability were extensively investigated. The results indicated that the CS-nHAC reinforcement materials can improve the corrosion resistance of the Mg matrix significantly and promote the proliferation and adhesion of mouse embryo osteoblast precursor cells (MC3T3-E1) and human umbilical vein endothelial cells (HUVECs). In addition, the CS-nHAC/Mg–Ca composites can not only promote the alkaline phosphatase (ALP) activity and extracellular matrix mineralization of MC3T3-E1 cells but also enhance the migration motility and vascular endothelial growth factor (VEGF) expression of HUVECs. Meanwhile, the 2CS-1nHAC/Mg–Ca composite exhibited the optimum function characteristics compared with other samples. Therefore, considering the improvement of corrosion resistance and biocompatibility, the CS-nHAC/Mg–Ca composites are expected to be a promising orthopedic implant.
“…The control group was a MAO Mg-Ca alloy (diameter 8 mm, height 2 mm, aperure 0.7 mm, 5 holes), as Mg-Ca. 33 (2) PMMA-nHAC composites preparation…”
Section: Methodsmentioning
confidence: 99%
“…After thorough mixing, PMMA bone cement was dip-coated on the surface of the prepared nHAC/Mg-Ca sheet at a rate of 2 m/s, and it was self-coagulated to form PMMA-nHAC/Mg-Ca, the schematic was as Figure 1. 33 The composite of each group are respectively denoted as Mg-Ca, PMMA-nHAC, PMMA-nHAC/Mg-Ca.Figure 1.Schematic diagram of composites preparation process and structure mode.…”
Section: Methodsmentioning
confidence: 99%
“…The control group was a MAO Mg-Ca alloy (diameter 8 mm, height 2 mm, aperure 0.7 mm, 5 holes), as Mg-Ca. 33 (2) PMMA-nHAC composites preparation Mineralized collagen powder (nHAC) was made of type I collagen and nano-hydroxyapatite, in which the volume of nano-hydroxyapatite was 45%-55% (Beijing Allgens Medical Science and Technology Co., Ltd., Beijing, China).…”
Section: Composites Preparation and Characterization Analysismentioning
confidence: 99%
“…32 In this study, nHAC was first compounded with magnesium-calcium alloy after micro-arc oxidation (MAO), and then a layer of PMMA bone cement was wrapped on the outer layer to strengthen the bonding force of the material, and our previous research has confirmed that PMMA/nHAC is compounded on Mg-Ca can effectively reduce the degradation rate of materials and improve surface hydrophilicity, promoted the adhesion, proliferation, differentiation, and good cell compatibility, osteogenic ability of MC3T3-E1. 33 In addition, osteogenesis is closely related to angiogenesis in the process of bone regeneration. As another decisive factor to promote bone integration, active angiogenesis not only improves the function of osteoblasts, but also improves the regeneration rate in bone tissue, especially in the process of early healing.…”
Magnesium (Mg) and its alloys show high degrees of biocompatibility and biodegradability, used as biodegrad able materials in biomedical applications. In this study, Polymethyl methacrylate (PMMA) - mineralized collagen (nano-Hydroxyapatite/collagen; nHAC)/Mg-Ca composite materials were prepared, to study the angiogenesis ability of its composite materials on Human umbilical vein endothelial cells (HUVECs) and its osteogenesis effect in vivo. The results showed that the PMMA-nHAC reinforcement materials can promote the proliferation and adhesion in HUVECs of Mg matrix significantly, it can enhance the migration motility and VEGF expression of HUVECs. In vivo, Micro-CT examination showed that with coated samples presenting the highest bone formation. Histologically, the materials and their corrosion products caused no systematic or local cytotoxicological effects. Therefore, the Mg matrix composites prepared in the present study has good biocompatibility and PMMA-nHAC/Mg-Ca composite may be an ideal orthopedic material to improve the bone formation, and biodegradable magnesium based implants with bioactivity have potential applications in bone tissue.
Owing to their excellent properties, magnesium alloys are widely used in bone tissue engineering. However, considerable work has been conducted to control the degradation rate and improve the cytocompatibility of magnesium alloys. In this study, low-cost production introduced a new bone repair composite (PCL-nHAC/Mg-Ca), which was composed of nano-hydroxylapatite-collagen (nHAC), polycaprolactone (PCL) and Mg-Ca alloy substrate treated by micro- arc oxidation (MAO). The experimental results showed that compared with the Mg-Ca alloy treated by MAO alone, the PCL-nHAC/Mg-Ca composite has a porous structure and a slower degradation rate. Cell experiments showed that the PCL-nHAC/Mg-Ca composite had good biocompatibility and significantly enhanced the proliferation of the MC3T3-E1 cells. The rabbit skull defect model further proved that the PCL-nHAC/Mg-Ca composite could regulate the degradation rate of the Mg-Ca alloy and promote the formation of bone tissue. Histological analyses showed that the PCL-nHAC/Mg-Ca composite had good stability in vivo and could better accelerate bone formation.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
