Study on Effect Elements of Exopolysaccharide Production of <i>Lactobacillus Kimchi</i> SR8 and DPPH Radical Scavenging Activity

Abstract

The correlation between exopolysaccharide production of Lactobacillus kimchi SR8 under different culture conditions and the DPPH radical scavenging activity of exopolysaccharide was studied. Lactobacillus kimchi SR8 produced 228.24 ± 2.23 mg/L exopolysaccharides with sucrose as the carbon source, Beef Extract-Peptone (2:1) as the nitrogen source and an initial pH of 6.50, whilst the DPPH radical scavenging activity of exopolysaccharides was only 6.85% ± 0.77% at a concentration of 0.20 mg/mL. However, the strain produced 206.79 ± 2.23 mg/L exopolysaccharides with glucose as the carbon source, Peptone-Tryptone (1:1) as the nitrogen source and an initial pH of 7.00, and the DPPH radical scavenging activity of exopolysaccharides increased to 28.34% ± 0.32% at the same concentration. The results showed no correlation between the exopolysaccharide production of lactic acid bacteria and the DPPH radical scavenging activity of exopolysaccharide. Therefore, the antioxidant activity should be taken into consideration when measures are taken to increase exopolysaccharide production if greater antioxidant activity of exopolysaccharides is preferred.

Probiotic Properties and Neuroprotective Effects of Lactobacillus buchneri KU200793 Isolated from Korean Fermented Foods

“…L. acidophilus ATCC 4356 showed 20.8% of DPPH radical scavenging activity in intracellular extract [29]. Antioxidative properties of LAB have been considered as strain-specific depending on the cell wall component, antioxidant enzymes, and exopolysaccharides [20,30]. A recent study referred that oxidative damage could initiate alpha-synuclein, which is a protein involved in Parkinson's disease [4].…”