Study on enzyme activity inhibition mechanism of Thiobacillus denitrification intracellular enzyme to sulfate-reducing bacteria intracellular enzyme

Abstract

Purpose Sulfate-reducing bacteria (SRB) are recognized by scholars as the most important class of bacteria leading to corrosion of metal materials. It is important to use the properties of microorganisms to inhibit the growth of SRB in the corrosion protection of metal materials and to protect the environment. Design/methodology/approach In this work, the behavior of anaerobic Thiobacillus denitrificans (TDN) intracellular enzyme inhibition of SRB corrosion of EH36 steel was investigated with electrochemical impedance spectroscopy, biological detection technology and X-ray photoelectron spectroscopy. Findings Results showed that the SRB crude intracellular enzyme affected the corrosion behavior of EH36 steel greatly and the purified TDN intracellular enzyme inhibits SRB intracellular enzyme corrosion to EH36 steel. Originality/value A perfect enzyme activity inhibition mechanism will provide theoretical guidance for the selection and application of anticorrosion microorganisms, which is of scientific significance in the field of microbial anticorrosion research.