Study on follicular fluid metabolomics components at different ages based on lipid metabolism

Abstract

Background: Follicular fluid is an important external environment for the growth and development of oocytes. A thorough identification of specific components in follicular fluid can better the existing understand of intracellular signal transduction and reveal potential biomarkers of oocyte health in women undergoing assisted reproductive therapy. To study on follicular fluid metabolomics components at different ages based on lipid metabolism, we have adopted a new method of SWATH to MRM(the sequential window acquisition of all theoretical fragment-ion spectra to multiple reaction monitor)metabolomics to provide extensive coverage and excellent quantitative data. This was done to investigate the differences in follicular fluid of patients undergoing in vitro fertilization (IVF) and embryo transfer in different age groups and to further explore the relationship between follicular fluid, age and reproductive function. Method: A combination of Ultra-high-performance liquid chromatography and high resolution mass spectrometry techniques were used to analyze the follicular fluid of 230 patients enrolled for the IVF cycle. The patients were of different ages grouped into two groups:the younger and older patients.The obtained multidimensional chromatographic data were processed by principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). The charge ratios and mass numbers enabled for the identification of different fragments in the samples. Matching information obtained through database search and the fragment information obtained by fragment ion scan structurally identified substances in the samples. This was used to determine the differential compounds. Results: The quality of oocytes decline with age,and the lipid composition in follicular fluid also changes,The lipid metabolism that changes with age may be related to the quality of oocytes.The main differences were in lipid metabolites. Some were up-regulated: Arachidonate, LysoPC(16:1), LysoPC(20:4) and LysoPC(20:3) while others were down-regulated: LysoPC(18:3) and LysoPC(18:1).

Follicular fluid and blood levels of persistent organic pollutants and reproductive outcomes among women undergoing assisted reproductive technologies

“…This reduced fertility can be due to the decline in total number of oocytes and their quality, as well as changes in the surrounding environment such as the FF. Previous studies have shown that possible age-related mechanisms include changes in the antioxidant defences and lipid metabolism in the FF (Luddi et al, 2020;Tatone et al, 2008;Zhang et al, 2020). Oxidative stress alters protein structures (Luddi et al, 2020;Tatone et al, 2008), which may in turn allows more chemicals to pass through the blood-follicle barrier.…”

The role of forskolin as a lipolytic stimulator during in vitro oocyte maturation and the in vitro embryo production of livestock

“…The progress of these innovative approaches could help to improving the optimization of mapping and quantifying genes, lipid and protein characterizations. Providing a new method to explore the lipidomic changes in oocyte and embryo development has open insights that hold potential to disentangle the function of lipid metabolism in embryo and oocyte developmental capability in animals (Chen et al., 2020; Zhang et al., 2020). Moreover, understanding the precise embryonic and oocyte lipid profile might contribute to improving the freezing protocol and enhance the cryotolerance of both oocyte and embryo (Zhang et al., 2020).…”

“…Providing a new method to explore the lipidomic changes in oocyte and embryo development has open insights that hold potential to disentangle the function of lipid metabolism in embryo and oocyte developmental capability in animals (Chen et al., 2020; Zhang et al., 2020). Moreover, understanding the precise embryonic and oocyte lipid profile might contribute to improving the freezing protocol and enhance the cryotolerance of both oocyte and embryo (Zhang et al., 2020). Further explorations are essential to explore the association between lipids and both embryo quality and sensitivity to cryopreservation in animals.…”

The Mechanism of Traditional Chinese Medicine Based on Semi-Targeted Metabolomics to Improve IVF Outcomes in Senile Patients

“…In the early stage, two projects of National Natural Science Foundation of China have used nontargeted follicular fluid metabolomics based on UHPLC-MS technology to preliminarily screen and explore the biomarkers of kidney qi deficiency syndrome in the elderly [ 15 ]. The study found that the components of follicular fluid in elder women were significantly different from that in younger women.…”