Agricultural tractor emissions have a notable influence on total emissions and climate change. This is especially important when developing an agricultural machinery emission inventory since tractors are the most widely used agricultural machinery. However, as with other agricultural machinery, there is a significant lack of data concerning tractors. This study combined two large-scale datasets to develop a Tier 2 tractor emission inventory for the Republic of Croatia. The first dataset, which was collected via a large-scale survey with 8895 respondents, includes data on fuel consumption by machine and engine type. The second dataset, which is official data of the Ministry of the Interior, includes data on machinery age. Since there is no direct link between datasets, data on fuel consumption were calculated for each of 21 Croatian counties and were then linked to the corresponding municipalities’ tractor age and power rating of the vehicle fleet. The result was used to calculate tractor emissions by with a Tier 2 approach using the method listed in the 2019 EMEP/EEA emissions guidebook. The calculations were made for five exhaust emission components: carbon dioxide (CO2), carbon monoxide (CO), hydrocarbon (HC), nitrogen oxide (NOX) and particulate matter (PM). The continental counties had the highest emissions for all emission types, especially the Osječko-baranjska and Vukovarsko-srijemska counties. The Tier 2 emission inventory was compared to data calculated using the Tier 1 method on a county level. There were notable differences between the inventories for HC and PM emissions, which is mainly due to Tier 2 approach having different emission factors depending on vehicle age. The end result was compared to the already existing Tier 1 emission inventory on a county level.