Study on in vitro micropropagation of Rosa sp.

Abstract

Tissue culture has long been recognized as an ideal experimental paradigm for studying the mechanisms governing plant cell growth, division, and physiological and biochemical processes. The present investigation was undertaken to establish a standard method for callus induction, shoot and root regeneration of rose at Genetic Engineering and Biotechnology Laboratory, University of Rajshahi, Bangladesh in 2010. Shoot tip and nodal segments were used as experimental materials excised from field grown plants for callus induction. The direct shoot was observed from both shoot tip and nodal segments for shoot regeneration. In vitro regenerated shoot cuttings were used for root regeneration. Proper manipulation of auxin (2, 4 -D and NAA) was used to induce callus from different explants. Different growth regulators (BAP, KIN and GA3) were casted-off in combination for shoot regeneration and proliferation. Again, different concentrations of auxins (IBA, NAA, and IAA) were applied for root initiation. Among the hormonal supplements used, 2, 4 -D was found best in all respect of callusing response for all types of explants. The highest percentage (90%) of callus induction was observed in media having MS (Murashige and Skoog) + 4.0 mg/L 2, 4 -D. In case of shoot regeneration, 100% of cultured explants regenerated shoot in media with MS + 2.0 mg/L BAP + 0.5 mg/L KIN. While, 2.0 mg/L BAP + 0.5 mg/L KIN + 0.1 mg/L GA3 induced 100% shoot proliferation. Moreover, for root induction, ½ MS + 1.0 mg/L IBA + 1.0 mg/L NAA proved to be the best (80%) from in vitro regenerated shoot cuttings, and the highest mean number of roots was 5.0. Rooted shoots were acclimatized and successfully established in a natural condition where 60% of the transplanted plants survived. Bangladesh J. Agri. 2022, 47(1): 66-74

Optimizing Micropropagation Protocol of Rose (Rosa hybrida L.) cv. ‘Double Delight’

“…The medium containing only 3.0 mg/l BAP resulted in the highest percentage of responding callus (93.33 %) out of all the different concentrations and combinations tested, with an average 6.40 ± 0.51 shoots per culture and an average of 5.48 ± 0.36 cm shoot length. Similarly, Afrin et al (2022) and Liu et al (2018) also demonstrated that BAP was crucial for the development of adventitious buds from callus in different species of Rosa. When BAP concentrations were raised or lowered after 3.0 mg/l, responsive callus was also generated, but the number of shoots/culture and their length were also reduced.…”

“…Moreover, the nature of callus obtained from different sources differed as well, as seen in Table 2. It was supported by the work of Harmon et al (2022) in the case of three different cultivars of R. hybrida; Afrin et al (2022) in the case of Rosa sp. ; Pourhosseini et al (2013) in the case of R. hybrida cv.…”