Study on microwave assisted extraction of chrysophanol and its intervention in biofilm formation of <i>Streptococcus suis</i>

Bai, Jingwen; Chen, Xingru; Tang, Yang; Cui, Weihong; Li, Da-Long; Bello-Onaghise, God’spower; Yang, Yu

doi:10.1039/c9ra04662c

Cited by 5 publications

(4 citation statements)

References 43 publications

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“…Figure 7(a) showed that SP significantly inhibited the biofilm formation of S. suis ( p < 0.05) at the concentrations of 1/2, 1/4 and 1/8 MIC, confirming that sub-inhibitory concentrations of SP effectively interfered with the biofilm formation of S. suis . Furthermore, SP displayed more efficient inhibitory effect on biofilm formation than rutin and chrysophanol (Bai et al , 2019; Liu et al , 2018). Therefore, SP might be used to treat the diseases spread through S. suis biofilms.…”

Section: Resultsmentioning

confidence: 99%

Separation, kinetics of purification of syringin by macroporous resin from Syringa oblata Lindl. leaves and the evaluation of bioactivities

Zhao

et al. 2021

PRT

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Purpose This paper aims to explore the adsorption kinetics of syringin from Syringa oblata Lindl. leaves on macroporous resin and develop an efficient, simple and recyclable technology for the separation and purification of syringin. Design/methodology/approach Static adsorption and desorption properties of six resins were tested to select a suitable resin for the purification of syringin. Langmuir and Freundlich isotherm models were used to estimate the adsorption behavior of syringin on AB-8 resin. Breakthrough point and eluent volume were determined by dynamic adsorption and desorption tests. High-performance liquid chromatography-electrospray ionization-mass spectrometry was applied to identify the syringin in the purified product [syringin product (SP)]. Antioxidant and antibacterial activities of SP in vitro were evaluated by free radical scavenging ability and biofilm formation inhibitory tests. Findings AB-8 exhibited the most suitable adsorption and desorption capacity. Adsorption isotherm parameters indicated favorable adsorption between AB-8 and syringin. The optimal results were as follows: for adsorption, the sample concentration was 1.85 mg/mL, the sample volume was 3.5 bed volume (BV), the flow rate was 0.5 mL/min; for desorption, the ethanol concentration was 70%, the elution volume was 2.5 BV, the elution velocity was 1.0 mL/min. SP with 80.28% syringin displayed the potent antioxidant activities and inhibitory effects on biofilm formation of Streptococcus suis. Originality/value To the best of authors’ knowledge, there are no reports on purifying syringin from Syringa oblata Lindl. leaves using macroporous resins. This paper may also provide a theoretical reference for the purification of other phenylpropanoid glucosides.

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Section: Resultsmentioning

confidence: 99%

Separation, kinetics of purification of syringin by macroporous resin from Syringa oblata Lindl. leaves and the evaluation of bioactivities

Zhao

et al. 2021

PRT

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“…Another phenolic compound found within Rheum palmatum that also demonstrates antibiofilm activity is chrysophanol ( 11 ). 67 This study reports inhibition of biofilm formation (around 60%) in Staphylococcus suis at concentrations as low as 0.112 μg mL −1 . SEM results demonstrated a single molecule state of the bacteria cells with a lack of interconnection to create a biofilm, however, the exact mechanism of antibiofilm action needs to be further explored.…”

Section: Plant-derived Natural Products With Antimicrobial Activitymentioning

confidence: 84%

Recent advances in the discovery of plant-derived antimicrobial natural products to combat antimicrobial resistant pathogens: insights from 2018–2022

et al. 2023

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“…It is noteworthy that drug residues in pig and human, multiple resistance, allergy, and gastrointestinal bleeding and other problems would arise after long-term application of common antibiotics [ 22 ]. Therefore, the development and utilization of natural products with antibacterial and antibiofilm properties is particularly urgent [ 23 , 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Syringopicroside Extracted from Syringa oblata Lindl on the Biofilm Formation of Streptococcus suis

et al. 2021

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Syringopicroside is a natural drug with antibacterial activity, which is the main ingredient of Syringa oblata Lindl (S. oblata). In order to further develop the application of S. oblata and evaluate the ability of syringopicroside against Streptococcus suis (S. suis), this investigation first applied an ultrasonic-assisted method to extract syringopicroside, and then response surface methodology (RSM) was performed to get the optimum condition. Based on RSM analysis, a second-order polynomial equation about the syringopicroside yield and four variables, including ultrasonic power, time, temperature, and liquid-to-solid ratio, was purposed. Through RSM prediction and model verification experiments, the optimum conditions were determined, as follows: ultrasonic time was 63 min, temperature was 60 °C, a liquid-to-solid ratio was set to 63 mL/g, and ultrasonic power was 835 W. Under this condition, a high syringopicroside yield was obtained (3.07 ± 0.13 mg/g), which was not significantly different with a predicated value. After separation and purification by HPD 500 microporous resin, then mass spectrum was applied to identify the main ingredient in aqueous extract. A minimal inhibitory concentration (MIC) assay revealed the value against S. suis of syringopicroside was 2.56 µg/µL and syringopicroside with sub-inhibitory concentrations that could effectively inhibit biofilm formation of S. suis. Besides, scanning electron microscopy analysis indicated syringopicroside could destroy the multi-layered aggregation structure of S. suis. Finally, molecular docking analysis confirmed that syringopicroside was combined with Orfy protein of S. suis through hydrogen bonds, hydrophobic interaction, and π-π stacking.

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Study on microwave assisted extraction of chrysophanol and its intervention in biofilm formation of Streptococcus suis

Abstract: A microwave assisted extraction technology was used to extract chrysophanol from rhubarb.

Cited by 5 publications

References 43 publications

Separation, kinetics of purification of syringin by macroporous resin from Syringa oblata Lindl. leaves and the evaluation of bioactivities

Separation, kinetics of purification of syringin by macroporous resin from Syringa oblata Lindl. leaves and the evaluation of bioactivities

Recent advances in the discovery of plant-derived antimicrobial natural products to combat antimicrobial resistant pathogens: insights from 2018–2022

Effect of Syringopicroside Extracted from Syringa oblata Lindl on the Biofilm Formation of Streptococcus suis

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