Study on Physiological Roles of Stimulation of Prostaglandin E₂ Receptor Subtype EP2 in Urethral Function in Rats

Abstract

Taken together, these results suggest that stimulation of EP2 receptors induces relaxation likely via activation of cAMP-dependent mechanisms in rat urethral tissue, leading to a reduction of UPP.

Effect of Selective Prostaglandin E2 EP2 Receptor Agonist CP‐533,536 on Voiding Efficiency in Rats with Midodrine‐Induced Functional Urethral Obstruction

“…CP‐533,536 has been reported as a selective EP2 receptor agonist with high affinity for the rat EP2 receptor with an IC 50 value of 50 nmol/L and 50‐fold more selectivity for EP2 than other PGE 2 ‐acting receptors, EP1, EP3 or EP4 . We previously reported that CP‐533,536 induces relaxation in rat urethral tissues pre‐contracted with the α 1 ‐adrenoceptor agonist phenylephrine . The present study showed that CP‐533,536 decreases UPP elevated by midodrine in rats.…”

“…14 We previously reported that CP-533,536 induces relaxation in rat urethral tissues pre-contracted with the α 1 -adrenoceptor agonist phenylephrine. 15 The present study showed that CP-533,536 decreases UPP elevated by midodrine in rats. Taken together, these findings suggest that a selective EP2 receptor agonist might decrease UPP by relieving urethral constriction caused by stimulation of α 1adrenoceptors.…”

“…Of these subtypes, EP2 and EP4 are generally associated with relaxation of smooth muscle . In our previous study, not only PGE 2 but also the selective EP2 receptor agonist CP‐533,536 relaxed rat urethral strips pre‐contracted with the α 1 ‐adrenoceptor agonist phenylephrine, suggesting that a selective EP2 receptor agonist might relieve urethral constriction.…”

Effect of ASP6432, a Novel Type 1 Lysophosphatidic Acid Receptor Antagonist, on Urethral Function and Prostate Cell Proliferation

“…Measurement of Urethral Perfusion Pressure. Urethral perfusion pressure (UPP) was measured according to a previously described method (Kurihara et al, 2016). In brief, female Wistar rats (Charles River Laboratories Japan Inc.) were anesthetized with an intraperitoneal injection of urethane (1.2 g/kg) and fixed in the supine position.…”

Urethral function and histopathology in aged female rats as a stress urinary incontinence model

“…The UPP in anesthetized rats was recorded continuously by infusing saline into the urethra at a rate of 4.5 mL/h. Following a stabilization period, UPP was measured in young and aged rats after intravenous administration of phenylephrine (0.03 mg/kg), which has been reported to elevate phenylephrine‐induced UPP . Changes in UPP (Δmm Hg) were calculated by subtracting baseline UPP just before phenylephrine administration from maximum UPP after phenylephrine (0.03 mg/kg, i.v.)…”