Study on polychlorobiphenyl serum levels in French consumers of freshwater fish

Intestinal exposure to PCB 153 induces inflammation via the ATM/NEMO pathway

“…To test the hypothesis that PCB 153 causes intestinal inflammation, mice were exposed to 300 μmol/kg of PCB 153 dissolved in safflower oil, delivered via oral gavage once per day for 2 days. This dosage was chosen based on typical amounts of PCBs found in fish meals of a highly polluted area and meant to represent the upper end of reported daily intake of PCBs (Desvignes et al 2015; Fromberg et al 2011). On the third day, mice were euthanized and IECs from the proximal small intestine, distal small intestine, and the colon were isolated.…”

“…Additionally, it is extremely challenging to accurately establish levels of dietary exposure to PCBs (Binnington et al 2016). Because of the mechanistic nature of this study, we chose to focus on only one congener, PCB 153, and levels of exposures that intestinal epithelial cells would encounter after a single meal high in PCBs (Desvignes et al 2015). While this dosage only represents a subset of the PCB exposed population, it was chosen in order to glean insight into the mechanism of the initial physiological response of IECs to an oral exposure to PCB 153.…”

“…Cells were exposed to either 50ng/mL LPS, 10μM or 1μM Etoposide, 100μM, 50μM, or 10μM PCB 153, or DMSO. Dosages of PCB 153 were chosen based on the range of possible concentrations that intestinal epithelial cells would be exposed to after a meal high in PCBs (Desvignes et al 2015). Cells were exposed for 3 hours and then collected for analysis.…”

“…Animals were exposed to 300 μmol/kg of PCB 153 stock solution dissolved in 100μL stripped safflower oil (Alfa Aesar, Ward Hill, MA) and administered via oral gavage. This dosage was chosen based on typical amounts of PCBs found in fish meals of a highly polluted area and meant to represent the high end of reported daily intake of PCBs in a single day (Desvignes et al 2015; Fromberg et al 2011). Control animals received an equal volume of DMSO as the PCB 153 stock solution, also dissolved in 100μL safflower oil (<1% DMSO by volume) For inhibition experiments, mice were intraperitoneally injected with PDTC (120mg/kg dissolved in 100uL of PBS) or vehicle (PBS), 30 minutes prior to PCB 153 exposure based on previously reported effective and nontoxic dosages (Lauzurica et al 1999).…”

Polychlorobiphenyls in freshwater fish: a new strategy to set maximum contamination limits

“…In 2015, Desvignes et al established a relationship between serum PCB concentrations and anglers' consumption of freshwater fish with high PCB bioaccumulation potential (Desvignes et al, 2015). This relationship was the basis to update recommendations with regard to safe consumption of freshwater fish.…”