Study on Quality Standards and Hepatoprotective Effect of Curcuma phaeocaulis Radix

Abstract

Curcuma phaeocaulis Radix (Lüsiyujin) is a mainstream variety of Curcumae Radix cultivated in Sichuan for more than 900 years, but its broader utility is limited by a lack of quality control and pharmacodynamic research. We used the Chinese Pharmacopoeia, 2015 edition, to guide the determination of germacrone and furanodienone content in extracts. We established an animal model of Qi stagnation and blood stasis in a clinical context. Pathological changes in liver function indexes were evaluated to investigate the hepatoprotective effect of Lüsiyujin. In 20 extraction batches of Lüsiyujin, ethanol extracts yielded 9.22%–15.01%, average 12.03%. The germacrone content was 0.003%–0.011% (average 0.0069%), and the furanodienone content was 0.090%–0.478% (average 0.201%). Compared with the control group, the model rats exhibited functional liver damage. Serum markers of liver function varied after treatment with Lüsiyujin, but significant effects were observed with alanine aminotransferase and total bile acid. This study demonstrates a simple method of quality control for Lüsiyujin. The study also demonstrated that Lüsiyujin inhibits hepatocyte damage and regulates the excretion and secretion of hepatic bile. Our findings provide a theoretical basis for the formulation of quality standards, clinical application, and resource development for the utilisation of Lüsiyujin.

Curcumae Radix: A Review of Traditional Use, Phytochemistry, Pharmacology, Toxicology and Quality Control

“…[132] In addition, CuR is able to protect against liver injury of the LDQS by inhibiting hepatocellular damage and regulating hepatic bile secretion and excretion. [133] In addition to its protective effects against the above types of liver injury, CuR has been shown to have ameliorative and interventional effects on liver fibrosis. It expresses anti-hepatic fibrosis and interventional hepatic fibrosis effects by eliminating free radicals, reducing inflammatory responses, inhibiting the proliferation of human hepatic stellate cells HCS-LX2 and the secretory function of fibrotic proteins, increasing the degradation of fibrotic proteins, antilipid peroxidation, anti-hepatic injury, and inhibiting the TGF-β1/Smad signaling pathway.…”

“…[224][225][226] Although there are fewer studies on the quality control of LSYJ and no fingerprints have been established, but Chen et al determined the content of ethanol extract, gimarone and furanodienone of 20 batches of LSYJ, which provided a simple quality control method for it. [133] Vinegar CuR and wine CuR are also widely used in clinical practice, and processing will enhance the analgesic and blood circulation effects of CuR. Therefore, it is necessary to select appropriate content determination indicators for the quality control of processed products.…”

Lvsiyujins A–G, new sesquiterpenoids, fromCurcuma phaeocaulisValeton root tuber and their preliminary pharmacological property assessment based on ADME evaluation, molecular docking andin vitroexperiments

“…There are relatively few studies on the chemical constituents of the root tuber of C. phaeocaulis (lvsiyujin), especially the study on the component separation, and only a few reports have identified some active components by chromatography-mass spectrometry. [7][8][9] Likewise, pharmacological research studies paid little attention to lvsiyujin, mainly focusing on the other three varieties. 2 In a previous research study, we have found that the extract of the rhizome, another tissue of C. phaeocaulis, could significantly inhibit the proliferation of MCF-7 cells.…”