Study on the degradation performance and kinetics of immobilized cells in straw-alginate beads in marine environment

Xue, Jianliang; Wu, Yanan; Shi, Ke; Xiao, Xinfeng; Gao, Yu; Lei, Lin; Qiao, Yanlu

doi:10.1016/j.biortech.2019.02.019

Cited by 86 publications

(20 citation statements)

References 25 publications

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“…Numerous current studies indicate that agricultural wastes can be used to immobilize carrier materials such as rice straw, peanut shell and biochar. 10,11 These materials have good capacity to transmit oxygen, hold water and improve the enzymatic activities. 12 Compared with raw agricultural wastes, biochar has high carbon content, high adsorption capacity, good stability, and optimal immobilization ability for bacteria and nutrients.…”

Section: Introductionmentioning

confidence: 99%

Bioremediation of petroleum hydrocarbon-contaminated soil by petroleum-degrading bacteria immobilized on biochar

Zhang

2019

RSC Adv.

115

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Section: Introductionmentioning

confidence: 99%

Bioremediation of petroleum hydrocarbon-contaminated soil by petroleum-degrading bacteria immobilized on biochar

Zhang

2019

RSC Adv.

115

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“…EPS with biosurfactant property, hereinafter named as surfactant exopolysaccharide or SEPS, find their role in several environmental applications like bioremediation by degradation of petroleum and its end products [14][15][16][17], microbial oil recovery [18,19], and biosorption of various toxic and heavy metals [20][21][22]. Rhamnolipid [23][24][25] produced by Pseudomonas aeruginosa or surfactin [26] produced by Bacillus subtilis are such SEPS, which have been applied in the aforesaid fields.…”

Section: Introductionmentioning

confidence: 99%

Exploring two contrasting surface‐active exopolysaccharides from a single strain of Ochrobactrum utilizing different hydrocarbon substrates

2019

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During production and characterization of exopolysaccharides (EPS) of Ochrobactrum pseudintermedium C1, it was observed that an experimental change in the basic hydrocarbon type of substrate for bacterial utilization led to elicitation of different surface-active properties in the EPS produced. In the sugar substrate, it elicited surfactant property, while in oil substrates it elicited emulsifying property, which indicated that the EPS might be different.Consequently, attention was focused on a detailed analysis of this substratespecific EPS. Utilizing waste sugar, edible, and mineral oil substrates, EPS produced in each situation was characterized. Besides estimating surface activity and thermostability, each substrate-specific EPS was analyzed by Fourier-transform infrared spectroscopy, gas chromatography-mass spectroscopy, 1 H-nuclear magnetic resonance, and matrix-assisted laser desorption/ ionization-time of flight mass spectroscopy to find any structural difference. The results were significantly contrasting although the similarity in molecular mass suggested a basic similarity in polysaccharide structure. Morphological differences were also evident both macroscopically and microscopically with scanning electron microscopy. As the surface-active property of EPS was dependent on the substrate utilized, their structural differences might account for it. These diverse surface activities of EPS produced by a single bacterial strain simply by changing the nature of substrate would also augment their bioapplications. Moreover, utilization of waste and easily available substrates should make such applications convenient, ecofriendly, and cost-worthy. K E Y W O R D S bacterial exopolysaccharide, chromophoric extracellular substance, contrasting surface activities, emulsifier exopolysaccharide, surfactant exopolysaccharide

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“…The Monod model is often used to compare the degradation kinetics of free and immobilised cells. This model describes the relationship between the degradation rate of a substrate and its initial concentration when the linear increase in the degradation rate starts at a value of zero [ 36 , 37 , 38 ]. However, the collected data indicated that in the systems with free cells without glucose and immobilised cells with glucose, there was a delay in degradation ( Figure 3 ).…”

Section: Resultsmentioning

confidence: 99%

“…Little is known about the kinetic parameters of paracetamol degradation by any other bacterial strains. In some cases, cell immobilisation clearly improved the efficiency of the xenobiotic degradation process [ 36 , 43 ]. Still, many reports show that immobilised cells degrade a given substance slower, but other aspects of the process, such as inhibitor tolerance, are improved [ 44 ].…”

Section: Resultsmentioning

confidence: 99%

Effect of Pseudomonas moorei KB4 Cells’ Immobilisation on Their Degradation Potential and Tolerance towards Paracetamol

2021

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Pseudomonas moorei KB4 is capable of degrading paracetamol, but high concentrations of this drug may cause an accumulation of toxic metabolites. It is known that immobilisation can have a protective effect on bacterial cells; therefore, the toxicity and degradation rate of paracetamol by the immobilised strain KB4 were assessed. Strain KB4 was immobilised on a plant sponge. A toxicity assessment was performed by measuring the concentration of ATP using the colony-forming unit (CFU) method. The kinetic parameters of paracetamol degradation were estimated using the Hill equation. Toxicity analysis showed a protective effect of the carrier at low concentrations of paracetamol. Moreover, a pronounced phenomenon of hormesis was observed in the immobilised systems. The obtained kinetic parameters and the course of the kinetic curves clearly indicate a decrease in the degradation activity of cells after their immobilisation. There was a delay in degradation in the systems with free cells without glucose and immobilised cells with glucose. However, it was demonstrated that the immobilised systems can degrade at least ten succeeding cycles of 20 mg/L paracetamol degradation. The obtained results indicate that the immobilised strain may become a useful tool in the process of paracetamol degradation.

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Study on the degradation performance and kinetics of immobilized cells in straw-alginate beads in marine environment

Cited by 86 publications

References 25 publications

Bioremediation of petroleum hydrocarbon-contaminated soil by petroleum-degrading bacteria immobilized on biochar

Bioremediation of petroleum hydrocarbon-contaminated soil by petroleum-degrading bacteria immobilized on biochar

Exploring two contrasting surface‐active exopolysaccharides from a single strain of Ochrobactrum utilizing different hydrocarbon substrates

Effect of Pseudomonas moorei KB4 Cells’ Immobilisation on Their Degradation Potential and Tolerance towards Paracetamol

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