Study on the expression of CK7, CK20 and other immunohistochemical markers for diagnosis of malignant tumours

Abstract

Introduction: Cancer is malignant disease, one of the leading cause of deadth at the global level. The determination of origin of tumors is really essential for not only prognosis but also effective treatment orientation for cancerous patients. Objective: Defining histopathological types of malignant epithelial tumors and determining the origin of tumors by CK7, CK20 and other immunohistochemical markers. Patients and methods: A cross-sectional study of 50 patients diagnosed carcinoma and CK7, CK20 and other immunohistochemical markers stain at Hue University of Medicine and Pharmacy Hospital from January, 2020 to December, 2020. Results: The results of histopathological types: lymph node metastatic carcinoma took up the highest percentage (30%), followed by lung cancer, hepatocellular cell carcinoma, gastric cancer and other organs with 28%, 18%, 10% and 2% respectively. The value of expression of CK7, CK20 and other immunohistochemical markers in differentiated diagnosis of the origin of tumors: CK7, CK20 positive 46%, 12% respectively. The combination of expression of CK7 and CK20: the co-expression of CK7 and CK20 with positive and negative was 2%, 44% respectively; the expression of CK7 positive and CK20 negative and vice versa was 44% and 10% respectively. CKAE1/3 with TTF1 (11/19), P63 (7/14), CEA (6/6), Ki-67 (13/2) and CK19 (10/6) had high positive percentage. Conclusion: Lymph node metastatic carcinoma was one of the most popular histopathological type. There was a statistically significant relationship between the expression of CK7 and CK20. The use of CK7, CK20 and other immunohistochemical markers can assist pathologists in determining exactly the origin of malignant tumors. Key words: carcinoma, CK7/CK20, histopathology, immunohistochemistry, metastasis.