Study on the In-vitro Culture Method for Normal Embryonic Cell Development of Porcine Parthenogenetic Embryos

Jung, Na-Hyeon; Kim, Sang‐Hwan; Kim, Dae-Seung; Yoon, Jong-Taek

doi:10.12750/jarb.35.1.94

Cited by 4 publications

(3 citation statements)

References 15 publications

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“…Taylor and his coworkers reported EVs in the maternal serum at 28-30 weeks of gestation (167). A recent in vitro study showed that the supplementation of IVC medium with pregnant sow serum inhibits apoptosis and promotes the cytoplasmic reorganization and differentiation of parthenogenetic embryos (168). Factors such as oxygen tension and glucose concentration regulate the release of EVs from the placenta.…”

Section: Placenta Derived Evs and Fetal Developmentmentioning

confidence: 99%

Extracellular Vesicle Mediated Crosstalk Between the Gametes, Conceptus, and Female Reproductive Tract

et al. 2020

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Extracellular vesicles (EVs) mediated intracellular communication plays an imperative role in the proper completion of different physiological events. Most of the bio-fluids are enriched with several subpopulations of EVs including exosomes and microvesicles (MVs), with the capacity of transferring different functional molecules (lipids, proteins, and nucleic acids) to target cells. Recipient cells upon receiving the signal molecules undergo different changes that positively affect the structural and functional integrity of the cells. This article was aimed to highlight the role of EVs secreted by gametes, the female reproductive tract, and the growing conceptus in the successful completion of different reproductive events related to gestation. EVs associated with the reproductive system are actively involved in the regulation of different physiological events including gamete maturation, fertilization, and embryo and fetal development. In the reproductive system, EVs mediated intracellular communication is not unidirectional but is rather regulated through crosstalk between the reproductive tract and the growing conceptus. These vesicles are secreted from the ovary, oviductal epithelium, endometrium, developing embryo, and the placenta. The cargo inside these vesicles exerts pleiotropic effects on both maternal and embryonic environments. A better understanding of the EVs-mediated crosstalk will be helpful in the development of useful tools serving both the diagnostic as well as therapeutic needs related to female fertility.

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Section: Placenta Derived Evs and Fetal Developmentmentioning

confidence: 99%

Extracellular Vesicle Mediated Crosstalk Between the Gametes, Conceptus, and Female Reproductive Tract

et al. 2020

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“…In the case of partheno embryos induced differentiation using FBS, the development rate of blastomere was relatively low, and particularly, it was confirmed that degradation were highly formed between the cytoplasm and the cell membrane. However, in the case of partheno embryos cultured using pregnency serum, it was confirmed that differentiation and fidelity of the cytoplasm increased (Jung et al, 2020).…”

Section: Discussionmentioning

confidence: 92%

Effects of pregnancy serum and scriptaid on development in early partheno embryo

Jung

Kim

et al. 2020

J Anim Reprod Biotechnol

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Partheno Embryo's research is known to play a very important role in identifying the development of embryonic cells or analyzing the genetic mechanisms of embryonic development, but the information on apoptosis formed during the early stage of development on Partheno Embryo is very little. Therefore, this study analyzed whether the embryonic cell death of unit embryos can be inhibited by adding Scriptaid, one of HDACi, which plays a role in demethylation of histone proteins as a method of regulating the cell cycle in the early embryo development of Partheno Embryo. As a result, the differentiation rate was higher in the group that added Scriptaid and FBS, but the cellular development was higher in the group that added pregnant serum to Scriptaid. As a result of analyzing the expression of the gene through IF and PCR, the group with the addition of gestational serum increased the expression of BCL2 and PCNA, which affects the anti-Casp3 action in cell survival. In addition, it is interpreted that treatment of Scriptaid for 16 hours, rather than 24 h treatment lowers the expression of Casp-3, a representative factor of apoptosis, and also increases embryonic development, thus affecting early embryo development. Therefore, it is concluded that the 16-hour treatment of Scriptaid and the use of gestational serum will inhibit cell death in the early embryonic development and increase the development rate of the embryo.

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“…In animals such as mice, ART is important for the conservation of genetic resources and the generation of genetically engineered animals (Hasegawa et al, 2017). Many ARTs have been developed, such as cryopreservation of sperm and embryos, superovulation, in vitro fertilization (IVF), embryo culture, and embryo transfer (ET) (Ha et al, 2013;Lee et al, 2015;Hasegawa et al, 2017;Lee et al, 2018;Barsuren et al, 2019;Chio et al, 2019;Kim and Park, 2019;Jung et al, 2020). The success of several ARTs such as cryo-preservation or IVF and the production of genetically engineered mice is dependent upon the ability of successful pregnancy after ET, therefore ET is an indispensable related to ART in mice (Lamas et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Hormone induced recipients for embryo transfer in mice

Lee

Kang

2021

J Anim Reprod Biotechnol

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Embryo transfer (ET) in the animal is an important procedure to generate genetically engineered animals and conserve genetic resources. For ET experiments in mice, pseudopregnant recipients are usually prepared with proestrus stage of females and vasectomized males. However, this conventional method is inefficient because the size of female colonies should be large to select only the proestrus stage in the estrous cycle and the surgical procedures are required to generate vasectomized males. In this study, we established a simple and efficient protocol to prepare ET recipients using the estrous synchronization with hormone injection and the mating with wild male mice. The delivery rate of ET recipients tended to be increased with estrous synchronization using hormone injection (100%) compared to the conventional method (71%). Further, natural pregnancy of the recipients, induced by mating with a wild male, significantly enhanced the birth rate of ET offspring than the conventional method (33% vs. 13%). Based on the results, we concluded that our new protocol using hormone injection to ET recipients and mating with wild males could be more efficient and simpler compared to the conventional method.

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Study on the In-vitro Culture Method for Normal Embryonic Cell Development of Porcine Parthenogenetic Embryos

Abstract: Studies of the parthenogenetic embryo can maintain the female's own genetic characteristics, in addition, it is used as an important data in various fields such as cytoplasmic activity, production of autologous stem cells,

Cited by 4 publications

References 15 publications

Extracellular Vesicle Mediated Crosstalk Between the Gametes, Conceptus, and Female Reproductive Tract

Extracellular Vesicle Mediated Crosstalk Between the Gametes, Conceptus, and Female Reproductive Tract

Effects of pregnancy serum and scriptaid on development in early partheno embryo

Hormone induced recipients for embryo transfer in mice

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