Study on the induction of somatic embryogenesis and morphological structural changes during the development of Handeliodendron bodinieri L.

Yu, Yaqing; Yang, Baolian; Ma, Daocheng; Guo, Song; Liao, Feifei; Li, Zailiu

doi:10.1007/s11240-023-02668-3

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2024

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Cited by 2 publications

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Callus Culture System from Lonicera japonica Thunb Anthers : Light Quality Effects on Callus Quality Evaluation

Cheng,

Guo,

Zhou

et al. 2024

Preprint

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Lonicera japonica Thunb has significant edible and medicinal value, offering properties like heat clearing, detoxification, antibacterial, and blood pressure reduction. However, its quality is limited by climate, environment, flowering period, and germplasm degradation. This study established the first embryogenic callus culture system for L. japonica anthers. Using abiotic inducers (light quality), the effects on callus morphology, metabolite accumulation, and antioxidant activity were examined. Results showed that adding 1.0 mg·L− 1 6-BA, 1.5 mg·L− 1 NAA, 1.5 mg·L− 1 2,4-D, and 0.2 mg·L− 1 KT to MS medium induced 81% embryogenic callus. Subculturing with 2.0 mg·L− 1 6-BA, 0.5 mg·L− 1 NAA, and 0.2 mg·L− 1 KT yielded uniform callus lines. SEM and paraffin sectioning distinguished callus types. After 15 days of blue light induction, active metabolites and antioxidant activities peaked. This system supports germplasm innovation and suggests abiotic inducers as a method for higher metabolic product yields.

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Callus Culture System from Lonicera japonica Thunb Anthers : Light Quality Effects on Callus Quality Evaluation

Cheng,

Guo,

Zhou

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

Establishment of <i>in vitro</i> regeneration system for leaves and petioles of <i>Oxalis triangularis</i> 'Purpurea'

Chen,

Xiao,

Wang

et al. 2024

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Study on the induction of somatic embryogenesis and morphological structural changes during the development of Handeliodendron bodinieri L.

Cited by 2 publications

References 57 publications

Callus Culture System from Lonicera japonica Thunb Anthers : Light Quality Effects on Callus Quality Evaluation

Callus Culture System from Lonicera japonica Thunb Anthers : Light Quality Effects on Callus Quality Evaluation

Establishment of <i>in vitro</i> regeneration system for leaves and petioles of <i>Oxalis triangularis</i> 'Purpurea'

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