Study on the Quality Evaluation of Compound Danshen Preparations Based on the xCELLigence Real-Time Cell-Based Assay and Pharmacodynamic Authentication

Yan, Guoquan; Zhu, Zhitao; Jin, Liliang; Xie, Hui; Miozzi, Jackelyn; Liu, Feifei; Wei, Xuchao; Jin-huo, Pan

doi:10.3390/molecules23092090

Cited by 9 publications

(6 citation statements)

References 18 publications

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“…This instrument has broad application prospects in various fields, such as drug screening, and development, toxicology analysis, pathology analysis, and herbal medicine. It plays an important role in the area of modern pharmaceutical evaluation and analysis [ 36 ]. It utilises patented microtiter plates (E-Plates ® ) which contain gold biosensors capable of monitoring the status of cells continuously and non-invasively in real time.…”

Section: Resultsmentioning

confidence: 99%

“…The system gives information about behaviour of cells, their adherence, proliferation and change in morphology, and expresses them in a dimensionless unit: cell index (CI). The greater the CI, the greater the adhesion of cells [ 36 , 37 ]. Introducing the effector to cell culture induces the response of the target adherent cells and the corresponding activity (loss of adherence), which can be detected with great sensitivity and precision.…”

Section: Resultsmentioning

confidence: 99%

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Analysis of Cytotoxicity of Selected Asteraceae Plant Extracts in Real Time, Their Antioxidant Properties and Polyphenolic Profile

et al. 2020

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Plants from Asteraceae family are widely used for their therapeutic effects in the treatment of gastrointestinal diseases, but the consequences of excessive intake still need to be studied. The aims of this study were the evaluation of cytotoxicity, measurement of antioxidant properties and determination of polyphenolic profile of Tanacetum vulgare L. (tansy), Achillea millefolium L. (yarrow) and Solidago gigantea Ait. (goldenrod) ethanolic extracts. The cytotoxicity of extracts was monitored by xCELLigence system in real time by using porcine intestinal epithelial cell line (IPEC-1) and by measurement of changes in metabolic activity ((3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (MTS) assay). The antioxidant properties were measured by spectrophotometric methods and polyphenolic profiles were determined by HPLC-DAD for 50% ethanol extracts (10% w/v). Strong cytotoxic effect was recorded for tansy and yarrow extracts (125–1000 µg/mL) by xCELLigence system and MTS assay. Conversely, a supportive effect on cell proliferation was recorded for goldenrod extracts (125 µg/mL) by the same methods (p < 0.001). The antioxidant activity was in good correlation with total polyphenolic content, and the highest value was recorded for goldenrod leaves, followed by tansy leaves, goldenrod flowers and yarrow leaf extracts. The goldenrod extracts were abundant with flavonoids, whereas phenolic acid derivatives predominated in the polyphenolic profile of tansy and yarrow.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Analysis of Cytotoxicity of Selected Asteraceae Plant Extracts in Real Time, Their Antioxidant Properties and Polyphenolic Profile

et al. 2020

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“…As a result, RTCA can track the cellular growth over the entire period of the experiment. Compared with the endpoint-based methods, using RTCA it is easier to notice the inhibition of cell growth and is especially effective in cell inhibitor research [14,15]. However, the correlation analysis between cell impedance detection and the classic toxic endpoint analysis was confirmed only in limited compounds, so there are some limitations for RTCA.…”

Section: Principles and Characteristicsmentioning

confidence: 99%

Application of Real-Time Cell Electronic Analysis System in Modern Pharmaceutical Evaluation and Analysis

Yan

Wei

et al. 2018

Molecules

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Objective: We summarized the progress of the xCELLigence real-time cell analysis (RTCA) technology application in recent years for the sake of enriching and developing the application of RTCA in the field of Chinese medicine. Background: The RTCA system is an established electronic cellular biosensor. This system uses micro-electronic biosensor technology that is confirmed for real-time, label-free, dynamic and non-offensive monitoring of cell viability, migration, growth, spreading, and proliferation. Methods: We summarized the relevant experiments and literature of RTCA technology from the principles, characteristics, applications, especially from the latest application progress. Results and conclusion: RTCA is attracting more and more attention. Now it plays an important role in drug screening, toxicology, Chinese herbal medicine and so on. It has wide application prospects in the area of modern pharmaceutical evaluation and analysis.

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“…The RTCA was employed to evaluate the interaction between cells and Mg-ACP/PAA nanoparticles. RTCA technology has emerged as an alternative non-invasive and label-free approach to assess cellular proliferation, cytotoxicity, and migration in real time at a cell-culture level [ 41 , 42 ]. The RTCA measures changes in impedance in a meshwork of interdigitated gold microelectrodes that are located at the well bottom (E-plate).…”

Section: Resultsmentioning

confidence: 99%

Biomimetic inorganic-organic hybrid nanoparticles from magnesium-substituted amorphous calcium phosphate clusters and polyacrylic acid molecules

Cui

Cong

et al. 2021

Bioactive Materials

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Amorphous calcium phosphate (ACP) has been widely found during bone and tooth biomineralization, but the meta-stability and labile nature limit further biomedical applications. The present study found that the chelation of polyacrylic acid (PAA) molecules with Ca 2+ ions in Mg-ACP clusters (~2.1 ± 0.5 nm) using a biomineralization strategy produced inorganic-organic Mg-ACP/PAA hybrid nanoparticles with better thermal stability. Mg-ACP/PAA hybrid nanoparticles (~24.0 ± 4.8 nm) were pH-responsive and could be efficiently digested under weak acidic conditions (pH 5.0–5.5). The internalization of assembled Mg-ACP/PAA nanoparticles by MC3T3-E1 cells occurred through endocytosis, indicated by laser scanning confocal microscopy and cryo-soft X-ray tomography. Our results showed that cellular lipid membranes remained intact without pore formation after Mg-ACP/PAA particle penetration. The assembled Mg-ACP/PAA particles could be digested in cell lysosomes within 24 h under weak acidic conditions, thereby indicating the potential to efficiently deliver encapsulated functional molecules. Both the in vitro and in vivo results preliminarily demonstrated good biosafety of the inorganic-organic Mg-ACP/PAA hybrid nanoparticles, which may have potential for biomedical applications.

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Study on the Quality Evaluation of Compound Danshen Preparations Based on the xCELLigence Real-Time Cell-Based Assay and Pharmacodynamic Authentication

Cited by 9 publications

References 18 publications

Analysis of Cytotoxicity of Selected Asteraceae Plant Extracts in Real Time, Their Antioxidant Properties and Polyphenolic Profile

Analysis of Cytotoxicity of Selected Asteraceae Plant Extracts in Real Time, Their Antioxidant Properties and Polyphenolic Profile

Application of Real-Time Cell Electronic Analysis System in Modern Pharmaceutical Evaluation and Analysis

Biomimetic inorganic-organic hybrid nanoparticles from magnesium-substituted amorphous calcium phosphate clusters and polyacrylic acid molecules

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